{"refrec":{"BRefID":231069,"RR":"<b>Lengger, S.K.; Hopmans, E.C.; Sinninghe Damsté, J.S.; Schouten, S.</b> (2012). Comparison of extraction and work up techniques for analysis of core and intact polar tetraether lipids from sedimentary environments. <i>Org. Geochem. 47</i>: 34-40. <a href=\"http://dx.doi.org/10.1016/j.orggeochem.2012.02.009\" target=\"_blank\">dx.doi.org/10.1016/j.orggeochem.2012.02.009</a>","BEntID":222781,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Org. Geochem. 47</i>: 34-40. <a href=\"https://dx.doi.org/10.1016/j.orggeochem.2012.02.009\" target=\"_blank\">https://dx.doi.org/10.1016/j.orggeochem.2012.02.009</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lengger, S.K.; Hopmans, E.C.; Sinninghe Damsté, J.S.; Schouten, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lengger, S.K. <i>et al.</i>","Englishabstract":"Glycerol dibiphytanyl glycerol tetraether-based intact polar lipids (IPL GDGTs) are used as biomarkers for living Archaea and are analyzed utilizing a variety of extraction and quantification techniques. Most IPL GDGT studies have used a modified Bligh-Dyer extraction method, but it has been suggested that Soxhlet extraction may be more efficient for environmental samples and biomass. We investigated the impact of three different extractions (Soxhlet, Bligh-Dyer and accelerated solvent extraction, ASE), two IPL quantification methods and two work up techniques (Na2SO4 and SiO2 column) on the amount and distribution of CL (core lipid)- and IPL-derived GDGTs and crenarchaeol-based IPLs in marine sediments from the Arabian Sea and Icelandic shelf, as well as a microbial mat from a Dutch beach. The different extraction procedures gave a similar yield of CL- and IPL-derived GDGTs. Direct analysis of crenarchaeol IPLs showed, however, that, while GDGTs with a monohexose head group were not affected by the extraction method, there was a large effect on IPL GDGTs containing dihexose or hexose, phosphohexose head groups. Quantification of IPL-derived GDGTs by way of either separation over a silica column or by subtraction of CL GDGTs in the total lipid extract before and after hydrolysis gave similar results, but the 'subtraction-method' had a relatively large quantification error. However, the silica column, as well as drying over a Na2SO4 column, resulted in a loss of the hexose, phosphohexose IPLs by up to 80%. Based on the results, a modified Bligh-Dyer extraction with as little further treatment as possible is recommended to allow measurement of the full range of IPL GDGTs in sediments.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Comparison of extraction and work up techniques for analysis of core and intact polar tetraether lipids from sedimentary environments","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2012,"MonPub":null,"DateUpdate":"2014-10-29","DateCreate":"2013-12-01","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000304853300004","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.orggeochem.2012.02.009"},"refs":null,"anarec":{"AnaID":231069,"PubliDate":2012,"Pagination":"34-40","XtraPublOfAnaID":null,"ISBN":null,"Volume":"47","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":111331,"SerRR":"Organic Geochemistry. Elsevier: Oxford; New York.  ISSN 0146-6380; e-ISSN 1873-5290","StandardTitleSer":"Organic Geochemistry","ISSN":"0146-6380","AbbrevSer":"Org. 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