{"refrec":{"BRefID":231239,"RR":"<b>Crawfurd, K.J.; Raven, J.A.; Wheeler, G.L.; Baxter, E.J.; Joint, I.</b> (2011). The response of <i>Thalassiosira pseudonana</i> to long-term exposure to increased CO<sub>2</sub> and decreased pH. <i>PLoS One 6(10)</i>: e26695. <a href=\"http://dx.doi.org/10.1371/journal.pone.0026695\" target=\"_blank\">dx.doi.org/10.1371/journal.pone.0026695</a>","BEntID":222951,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>PLoS One 6(10)</i>: e26695. <a href=\"https://dx.doi.org/10.1371/journal.pone.0026695\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0026695</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Crawfurd, K.J.; Raven, J.A.; Wheeler, G.L.; Baxter, E.J.; Joint, I.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Crawfurd, K.J. <i>et al.</i>","Englishabstract":"The effect of ocean acidification conditions has been investigated in cultures of the diatom <i>Thalassiosira pseudonana</i> CCMP1335. Expected end-of-the-century pCO<sub>2</sub> (aq) concentrations of 760 µatm (equivalent to pH 7.8) were compared with present-day condition (380 µatm CO<sub>2</sub>, pH 8.1). Batch culture pH changed rapidly because of CO<sub>2</sub> (aq) assimilation and pH targets of 7.8 and 8.1 could not be sustained. Long-term (∼100 generation) pH-auxostat, continuous cultures could be maintained at target pH when cell density was kept low (<2×10<sup>5</sup> cells mL<sup>−1</sup>). After 3 months continuous culture, the C:N ratio was slightly decreased under high CO<sub>2</sub> conditions and red fluorescence per cell was slightly increased. However, no change was detected in photosynthetic efficiency (F<sub>v</sub>/F<sub>m</sub>) or functional cross section of PS II (σ<sub>PSII</sub>). Elevated pCO<sub>2</sub> has been predicted to be beneficial to diatoms due to reduced cost of carbon concentration mechanisms. There was reduced transcription of one putative δ-carbonic anhydrase (<i>CA-4</i>) after 3 months growth at increased CO<sub>2</sub> but 3 other δ-CAs and the small subunit of RUBISCO showed no change. There was no evidence of adaptation or clade selection of <i>T. pseudonana</i> after ∼100 generations at elevated CO<sub>2</sub>. On the basis of this long-term culture, pH change of this magnitude in the future ocean may have little effect on <i>T. pseudonana</i> in the absence of genetic adaption.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The response of <i>Thalassiosira pseudonana</i> to long-term exposure to increased CO<sub>2</sub> and decreased pH","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2011,"MonPub":null,"DateUpdate":"2016-09-21","DateCreate":"2013-12-01","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000299081800027","VABBcode":null,"OpenAcc":1,"DOI":"10.1371/journal.pone.0026695"},"refs":null,"anarec":{"AnaID":231239,"PubliDate":2011,"Pagination":"e26695","XtraPublOfAnaID":null,"ISBN":null,"Volume":"6","Issue":"10","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":123954,"SerRR":"PLoS One. Public Library of Science: San Francisco.  ISSN 1932-6203; e-ISSN 1932-6203","StandardTitleSer":"PLoS One","ISSN":"1932-6203","AbbrevSer":"PLoS One","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Crawfurd","Firstname":null,"Initials":"K.J.","Affiliation":"Plymouth Marine Lab, Plymouth, Devon, England.","Discriminator":null,"CorporateFlag":0,"BEntID":222951,"AutID":172452,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Raven","Firstname":null,"Initials":"J.A.","Affiliation":"Univ Dundee, Div Plant Sci, James Hutton Inst, Dundee,Scotland.","Discriminator":null,"CorporateFlag":0,"BEntID":222951,"AutID":172453,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Wheeler","Firstname":null,"Initials":"G.L.","Affiliation":"Plymouth Marine Lab, Plymouth, Devon, England.","Discriminator":null,"CorporateFlag":0,"BEntID":222951,"AutID":172454,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Baxter","Firstname":null,"Initials":"E.J.","Affiliation":"Plymouth Marine Lab, Plymouth, Devon, England.","Discriminator":null,"CorporateFlag":0,"BEntID":222951,"AutID":172455,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Joint","Firstname":null,"Initials":"I.","Affiliation":"Plymouth Marine Lab, Plymouth, Devon, England.","Discriminator":null,"CorporateFlag":0,"BEntID":222951,"AutID":172456,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":295315,"LibID":36,"BRefID":231239,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"295315","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Public Library of Science","City":"San Francisco"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":222951,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":222951,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1371/journal.pone.0026695","externalID":"10.1371/journal.pone.0026695","URLTypeCode":"DOI","URLID":30865,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2013-12-01 18:55:58.187000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2016-09-21 12:30:32.020000","timezone_type":3,"timezone":"Europe/Brussels"}}}