{"refrec":{"BRefID":231276,"RR":"<b>Hackenberg, C.; Kern, R.; Huge, J.; Stal, L.J.; Tsuji, Y.; Kopka, J.; Shiraiwa, Y.; Bauwe, H.; Hagemann, M.</b> (2011). Cyanobacterial lactate oxidases serve as essential partners in N<sub>2</sub> fixation and evolved into photorespiratory glycolate oxidases in plants. <i>Plant Cell 23(8)</i>: 2978-2990. <a href=\"http://dx.doi.org/10.1105/tpc.111.088070\" target=\"_blank\">dx.doi.org/10.1105/tpc.111.088070</a>","BEntID":222988,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Plant Cell 23(8)</i>: 2978-2990. <a href=\"https://dx.doi.org/10.1105/tpc.111.088070\" target=\"_blank\">https://dx.doi.org/10.1105/tpc.111.088070</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Hackenberg, C.; Kern, R.; Huge, J.; Stal, L.J.; Tsuji, Y.; Kopka, J.; Shiraiwa, Y.; Bauwe, H.; Hagemann, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Hackenberg, C. <i>et al.</i>","Englishabstract":"Glycolate oxidase (GOX) is an essential enzyme involved in photorespiratory metabolism in plants. In cyanobacteria and green algae, the corresponding reaction is catalyzed by glycolate dehydrogenases (GlcD). The genomes of N<sub>2</sub>-fixing cyanobacteria, such as <i>Nostoc</i> PCC 7120 and green algae, appear to harbor genes for both GlcD and GOX proteins. The GOX-like proteins from <i>Nostoc</i> (No-LOX) and from <i>Chlamydomonas reinhardtii</i> showed high <span class=\"sc\">l</span>-lactate oxidase (LOX) and low GOX activities, whereas glycolate was the preferred substrate of the phylogenetically related At-GOX2 from <i>Arabidopsis thaliana</i>. Changing the active site of No-LOX to that of At-GOX2 by site-specific mutagenesis reversed the LOX/GOX activity ratio of No-LOX. Despite its low GOX activity, No-LOX overexpression decreased the accumulation of toxic glycolate in a cyanobacterial photorespiratory mutant and restored its ability to grow in air. A LOX-deficient <i>Nostoc</i> mutant grew normally in nitrate-containing medium but died under N<sub>2</sub>-fixing conditions. Cultivation under low oxygen rescued this lethal phenotype, indicating that N<sub>2</sub> fixation was more sensitive to O<sub>2</sub> in the Δ<i>lox Nostoc</i> mutant than in the wild type. We propose that LOX primarily serves as an O<sub>2</sub>-scavenging enzyme to protect nitrogenase in extant N<sub>2</sub>-fixing cyanobacteria, whereas in plants it has evolved into GOX, responsible for glycolate oxidation during photorespiration.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Cyanobacterial lactate oxidases serve as essential partners in N<sub>2</sub> fixation and evolved into photorespiratory glycolate oxidases in plants","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2011,"MonPub":null,"DateUpdate":"2016-09-22","DateCreate":"2013-12-01","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000295254700016","VABBcode":null,"OpenAcc":0,"DOI":"10.1105/tpc.111.088070"},"refs":null,"anarec":{"AnaID":231276,"PubliDate":2011,"Pagination":"2978-2990","XtraPublOfAnaID":null,"ISBN":null,"Volume":"23","Issue":"8","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":59330,"SerRR":"The Plant Cell. 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