{"refrec":{"BRefID":231309,"RR":"<b>Peperzak, L.; Brussaard, C.P.D.</b> (2011). Flow Cytometric Applicability of Fluorescent Vitality Probes on Phytoplankton. <i>J. Phycol. 47(3)</i>: 692-702. <a href=\"http://dx.doi.org/10.1111/j.1529-8817.2011.00991.x\" target=\"_blank\">dx.doi.org/10.1111/j.1529-8817.2011.00991.x</a>","BEntID":223021,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>J. Phycol. 47(3)</i>: 692-702. <a href=\"https://dx.doi.org/10.1111/j.1529-8817.2011.00991.x\" target=\"_blank\">https://dx.doi.org/10.1111/j.1529-8817.2011.00991.x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Peperzak, L.; Brussaard, C.P.D.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Peperzak, L.; Brussaard, C.P.D.","Englishabstract":"The applicability of six fluorescent probes (four esterase probes: acetoxymethyl ester of Calcein [Calcein-AM], 5-chloromethylfluorescein diacetate [CMFDA], fluorescein diacetate [FDA], and 2',7'-dichlorofluorescein diacetate [H(2)DCFDA]; and two membrane probes: bis-(1,3-dibutylbarbituric acid) trimethine oxonol [DiBAC(4)(3)] and SYTOX-Green) as vitality stains was tested on live and killed cells of 40 phytoplankton strains in exponential and stationary growth phases, belonging to 12 classes and consisting of four cold-water, 26 temperate, and four warm-water species. The combined live/dead ratios of all six probes indicated significant differences between the 12 plankton classes (P < 0.01) and between individual species (P < 0.05). No specific differences were observed among strains of one species, among species or strains from different origin, nor between cells in exponential and stationary growth phase except for FDA. FDA showed a significant (P < 0.05) drop of <20% in fluorescence intensity in stationary cells. Of the four esterase probes, the live/dead ratios of FDA and CMFDA were not significantly different from each other, and both performed better than Calcein-AM and H(2)DCFDA (P < 0.001). Of the two membrane probes, DIBAC(4)(3) stained rhodophytes and euglenophytes much better than SYTOX-Green. The 13 algal strains best stainable (high live/dead ratios) among all six probes belonged to,nine genera from six classes of phytoplankton. In conclusion, FDA, CMFDA, DIBAC(4)(3), and SYTOX-Green represent a wide choice of vitality probes in the study of phytoplankton ecology, applicable in many species from different algal classes, originating from different regions and at different stages of growth.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Flow Cytometric Applicability of Fluorescent Vitality Probes on Phytoplankton","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Calcein-AM; CMFDA (CellTracker Green); DiBAC(4)(3); FDA; flow cytometry;H(2)DCFDA; live/dead assay; phytoplankton; SYTOX-GREEN; vitality","OtherDescriptors":null,"Notes":null,"AnaPub":2011,"MonPub":null,"DateUpdate":"2016-06-15","DateCreate":"2013-12-01","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000292064000027","VABBcode":null,"OpenAcc":0,"DOI":"10.1111/j.1529-8817.2011.00991.x"},"refs":null,"anarec":{"AnaID":231309,"PubliDate":2011,"Pagination":"692-702","XtraPublOfAnaID":null,"ISBN":null,"Volume":"47","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43251,"SerRR":"Journal of Phycology. Blackwell Science: New York.  ISSN 0022-3646; e-ISSN 1529-8817","StandardTitleSer":"Journal of Phycology","ISSN":"0022-3646","AbbrevSer":"J. Phycol.","StandardTitleMon":null,"StartPage":692,"Pages":11,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Peperzak","Firstname":"Louis","Initials":"L.","Affiliation":"Department of Biological Oceanography, Royal Netherlands Institute for Sea Research / NIOZ","Discriminator":null,"CorporateFlag":0,"BEntID":223021,"AutID":172816,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"BIO","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; NIOZ Texel; Afdeling Biologische Oceanografie","ORCID":"0000-0003-0691-2521","PersID":29210,"InsID":13080},{"AutName":"Brussaard","Firstname":"Corina","Initials":"C.P.D.","Affiliation":"Department of Biological Oceanography, Royal Netherlands Institute for Sea Research / NIOZ","Discriminator":null,"CorporateFlag":0,"BEntID":223021,"AutID":175426,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"BIO","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; NIOZ Texel; Afdeling Biologische Oceanografie","ORCID":null,"PersID":10995,"InsID":13080}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":223021,"BEntPrivate":null,"availability":[{"BInstID":255210,"LibID":2779,"BRefID":231309,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"255210","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":261,"Classification":"NIOZ files","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":null,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":805,"SERpubls":[{"PublName":"Blackwell Science","City":"New York"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"805":{"SpName":"Koninklijk Nederlands Instituut voor Onderzoek der Zee","SpColID":805,"ParSpColID":null,"TopParID":null,"ShortName":"NIOZ","URLLocation":"https://www.vliz.be/imis/nioz/imis.php?refid=","LibID":2779,"OpenRepoFlag":1,"SpTypID":1,"TopParIDNotWebsite":null,"SpColPath":"NIOZ"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":223021,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":223021,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1111/j.1529-8817.2011.00991.x","externalID":"10.1111/j.1529-8817.2011.00991.x","URLTypeCode":"DOI","URLID":30933,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2013-12-01 18:55:58.187000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Marlies.Bruining@nioz.nl","updSesDate":{"date":"2016-06-15 14:07:53.370000","timezone_type":3,"timezone":"Europe/Brussels"}}}