{"refrec":{"BRefID":238266,"RR":"<b>Hollants, J.; Leliaert, F.; Verbruggen, H.; De Clerck, O.; Willems, A.</b> (2013). Host specificity and coevolution of Flavobacteriaceae endosymbionts within the siphonous green seaweed <i>Bryopsis</i>. <i>Mol. Phylogenet. Evol. 67(3)</i>: 608-614. <a href=\"http://dx.doi.org/10.1016/j.ympev.2013.02.025\" target=\"_blank\">dx.doi.org/10.1016/j.ympev.2013.02.025</a>","BEntID":229953,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Mol. Phylogenet. Evol. 67(3)</i>: 608-614. <a href=\"https://dx.doi.org/10.1016/j.ympev.2013.02.025\" target=\"_blank\">https://dx.doi.org/10.1016/j.ympev.2013.02.025</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Hollants, J.; Leliaert, F.; Verbruggen, H.; De Clerck, O.; Willems, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Hollants, J. <i>et al.</i>","Englishabstract":"The siphonous green seaweed <i>Bryopsis</i> harbors complex intracellular bacterial communities. Previous studies demonstrated that certain species form close, obligate associations with Flavobacteriaceae. A predominant imprint of host evolutionary history on the presence of these bacteria suggests a highly specialized association. In this study we elaborate on previous results by expanding the taxon sampling and testing for host–symbiont coevolution Therefore, we optimized a PCR protocol to directly and specifically amplify Flavobacteriaceae endosymbiont 16S rRNA gene sequences, which allowed us to screen a large number of algal samples without the need for cultivation or surface sterilization. We analyzed 146 <i>Bryopsis</i> samples, and 92 additional samples belonging to the Bryopsidales and other orders within the class Ulvophyceae. Results indicate that the Flavobacteriaceae endosymbionts are restricted to <i>Bryopsis</i>, and only occur within specific, warm-temperate and tropical clades of the genus. Statistical analyses (AMOVA) demonstrate a significant non-random host–symbiont association. Comparison of bacterial 16S rRNA and <i>Bryopsis rbc</i>L phylogenies, however, reveal complex host–symbiont evolutionary associations, whereby closely related hosts predominantly harbor genetically similar endosymbionts. Bacterial genotypes are rarely confined to a single <i>Bryopsis</i> species and most <i>Bryopsis</i> species harbored several Flavobacteriaceae, obscuring a clear pattern of coevolution.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Host specificity and coevolution of Flavobacteriaceae endosymbionts within the siphonous green seaweed <i>Bryopsis</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Alga; Bacteria; Coevolution; Codivergence; Endosymbiosis</td>","OtherDescriptors":null,"Notes":null,"AnaPub":2013,"MonPub":null,"DateUpdate":"2015-10-29","DateCreate":"2014-05-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000317996100006","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.ympev.2013.02.025"},"refs":null,"anarec":{"AnaID":238266,"PubliDate":2013,"Pagination":"608-614","XtraPublOfAnaID":null,"ISBN":null,"Volume":"67","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45071,"SerRR":"Molecular Phylogenetics and Evolution. 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