{"refrec":{"BRefID":238312,"RR":"<b>Orts, D.J.B.; Peigneur, S.; Madio, B.; Cassoli, J.S.; Montandon, G.G.; Pimenta, A.M.C.; Bicudo, J.E.P.W.; Freitas, J.C.; Zaharenko, A.J.; Tytgat, J.</b> (2013). Biochemical and electrophysiological characterization of two sea anemone Type 1 Potassium toxins from a geographically distant population of <i>Bunodosoma caissarum</i>. <i>Mar. Drugs 11(3)</i>: 655-679. <a href=\"http://dx.doi.org/10.3390/md11030655\" target=\"_blank\">dx.doi.org/10.3390/md11030655</a>","BEntID":229999,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Drugs 11(3)</i>: 655-679. <a href=\"https://dx.doi.org/10.3390/md11030655\" target=\"_blank\">https://dx.doi.org/10.3390/md11030655</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Orts, D.J.B.; Peigneur, S.; Madio, B.; Cassoli, J.S.; Montandon, G.G.; Pimenta, A.M.C.; Bicudo, J.E.P.W.; Freitas, J.C.; Zaharenko, A.J.; Tytgat, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Orts, D.J.B. <i>et al.</i>","Englishabstract":"Sea anemone (Cnidaria, Anthozoa) venom is an important source of bioactive compounds used as tools to study the pharmacology and structure-function of voltage-gated K<sup>+</sup> channels (K<sub>V</sub>). These neurotoxins can be divided into four different types, according to their structure and mode of action. In this work, for the first time, two toxins were purified from the venom of <i>Bunodosoma caissarum</i> population from Saint Peter and Saint Paul Archipelago, Brazil. Sequence alignment and phylogenetic analysis reveals that BcsTx1 and BcsTx2 are the newest members of the sea anemone type 1 potassium channel toxins. Their functional characterization was performed by means of a wide electrophysiological screening on 12 different subtypes of K<sub>V </sub>channels (K<sub>V</sub>1.1–K<sub>V</sub>1.6; K<sub>V</sub>2.1; K<sub>V</sub>3.1; K<sub>V</sub>4.2; K<sub>V</sub>4.3; <i>h</i>ERG and <i>Shaker </i>IR). BcsTx1 shows a high affinity for rKv1.2 over rKv1.6, hKv1.3, <i>Shaker</i> IR and rKv1.1, while Bcstx2 potently blocked rKv1.6 over hKv1.3, rKv1.1, <i>Shaker</i> IR and rKv1.2. Furthermore, we also report for the first time a venom composition and biological activity comparison between two geographically distant populations of sea anemones.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Biochemical and electrophysiological characterization of two sea anemone Type 1 Potassium toxins from a geographically distant population of <i>Bunodosoma caissarum</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"sea anemone; Bunodosoma caissarum; neurotoxins; voltage-gated potassiumchannels; two-electrode voltage-clamp; Xenopus laevis; intraspecificvenom variation; Saint Peter and Saint Paul Archipelago","OtherDescriptors":null,"Notes":null,"AnaPub":2013,"MonPub":null,"DateUpdate":"2014-05-28","DateCreate":"2014-05-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000316607800007","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/md11030655"},"refs":null,"anarec":{"AnaID":238312,"PubliDate":2013,"Pagination":"655-679","XtraPublOfAnaID":null,"ISBN":null,"Volume":"11","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":113252,"SerRR":"Marine Drugs. 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