{"refrec":{"BRefID":238372,"RR":"<b>Delroisse, J.; Lanterbecq, D.; Eeckhaut, I.; Mallefet, J.; Flammang, P.</b> (2013). Opsin detection in the sea urchin <i>Paracentrotus lividus</i> and the sea star <i>Asterias rubens</i>. <i>Cah. Biol. Mar. 54(4)</i>: 721-727","BEntID":230059,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Cah. Biol. Mar. 54(4)</i>: 721-727","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Delroisse, J.; Lanterbecq, D.; Eeckhaut, I.; Mallefet, J.; Flammang, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Delroisse, J. <i>et al.</i>","Englishabstract":"Vision in metazoans is permitted by opsin expression in photoreceptor cells. These opsins can be mainly classified into two groups: the ciliary (c) and the rhabdomeric (r) opsins. Based on the r-opsin sequence of the sea urchin <i>Strongylocentrotus purpuratus</i>, degenerate primers were designed and used to amplify homologous opsin genes in the sea urchin <i>Paracentrotus lividus</i> and the sea star <i>Asterias rubens</i> by PCR. Partial r-opsin sequences were obtained for both species. These sequences are more similar between sea urchin species than between sea urchins and sea stars. In parallel, a commercial antibody, raised against the N-terminal domain of the rat rhodopsin, was used to detect c-opsins in Western blot experiments. Putative c-opsins were detected in the oral integument of <i>P. lividus</i>. We also detected c-opsins in the aboral integument of <i>A. rubens</i>, but not in the podia nor in the optic cushions. In all eventualities, it is therefore likely that two types of opsins co-occur in <i>A. rubens</i> and <i>P. lividus</i> as it is the case in <i>S. purpuratus</i>. In <i>A. rubens</i>, one opsin type would be located in the optic cushions (r-opsins) and another in the aboral integument (c-opsin). This could support the idea that sea stars possess two types of vision: the optic cushions, involved in visual perception of the environment, and the aboral integument, involved in non-visual photoreception.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Opsin detection in the sea urchin <i>Paracentrotus lividus</i> and the sea star <i>Asterias rubens</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Echinoderms; Sea-urchins; Sea-stars; Photoreception; Opsin</td>","OtherDescriptors":null,"Notes":null,"AnaPub":2013,"MonPub":null,"DateUpdate":"2014-06-12","DateCreate":"2014-05-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000326954200033","VABBcode":null,"OpenAcc":1},"refs":null,"anarec":{"AnaID":238372,"PubliDate":2013,"Pagination":"721-727","XtraPublOfAnaID":null,"ISBN":null,"Volume":"54","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42348,"SerRR":"Cahiers de Biologie Marine. 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