{"refrec":{"BRefID":238541,"RR":"<b>Ciancia, M.; Alberghina, J.; Arata, P.X.; Benavides, H.; Leliaert, F.; Verbruggen, H.; Estevez, J.M.</b> (2012). Characterization of cell wall polysaccharides of the coenocytic green seaweed <i>Bryopsis plumosa</i> (Bryopsidaceae, Chlorophyta) from the Argentine coast. <i>J. Phycol. 48(2)</i>: 326-335. <a href=\"http://dx.doi.org/10.1111/j.1529-8817.2012.01131.x\" target=\"_blank\">dx.doi.org/10.1111/j.1529-8817.2012.01131.x</a>","BEntID":230228,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>J. Phycol. 48(2)</i>: 326-335. <a href=\"https://dx.doi.org/10.1111/j.1529-8817.2012.01131.x\" target=\"_blank\">https://dx.doi.org/10.1111/j.1529-8817.2012.01131.x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ciancia, M.; Alberghina, J.; Arata, P.X.; Benavides, H.; Leliaert, F.; Verbruggen, H.; Estevez, J.M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ciancia, M. <i>et al.</i>","Englishabstract":"<i>Bryopsis</i> sp. from a restricted area of the rocky shore of Mar del Plata (Argentina) on the Atlantic coast was identified as <i>Bryopsis plumosa</i> (Hudson) C. Agardh (Bryopsidales, Chlorophyta) based on morphological characters and <i>rbc</i>L and <i>tuf</i>A DNA barcodes. To analyze the cell wall polysaccharides of this seaweed, the major room temperature (B1) and 90°C (X1) water extracts were studied. By linkage analysis and NMR spectroscopy, the structure of a sulfated galactan was determined, and putative sulfated rhamnan structures and furanosidic nonsulfated arabinan structures were also found. By anion exchange chromatography of X1, a fraction (F4), comprising a sulfated galactan as major structure was isolated. Structural analysis showed a linear backbone constituted of 3-linked ß-<span class=\"smallCaps\">d</span>-galactose units, partially sulfated on C-6 and partially substituted with pyruvic acid forming an acetal linked to <i>O</i>-4 and <i>O</i>-6. This galactan has common structural features with those of green seaweeds of the genus <i>Codium</i> (Bryopsidales, Chlorophyta), but some important differences were also found. This is the first report about the structure of the water-soluble polysaccharides biosynthesized by seaweeds of the genus <i>Bryopsis</i>. These sulfated galactans and rhamnans were in situ localized mostly in two layers, one close to the plasma membrane and the other close to the apoplast, leaving a middle amorphous, unstained cell wall zone. In addition, fibrillar polysaccharides, comprising (1?3)-ß-<span class=\"smallCaps\">d</span>-xylans and cellulose, were obtained by treatment of the residue from the water extractions with an LiCl/DMSO solution at high temperature. These polymers were also localized in a bilayer arrangement.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Characterization of cell wall polysaccharides of the coenocytic green seaweed <i>Bryopsis plumosa</i> (Bryopsidaceae, Chlorophyta) from the Argentine coast","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Bryopsis plumose; cell wall; green seaweed; pyruvylated galactansulfate; sulfated polysaccharides","OtherDescriptors":null,"Notes":null,"AnaPub":2012,"MonPub":null,"DateUpdate":"2015-10-29","DateCreate":"2014-05-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000302467900009","VABBcode":null,"OpenAcc":0,"DOI":"10.1111/j.1529-8817.2012.01131.x"},"refs":null,"anarec":{"AnaID":238541,"PubliDate":2012,"Pagination":"326-335","XtraPublOfAnaID":null,"ISBN":null,"Volume":"48","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43251,"SerRR":"Journal of Phycology. Blackwell Science: New York.  ISSN 0022-3646; e-ISSN 1529-8817","StandardTitleSer":"Journal of Phycology","ISSN":"0022-3646","AbbrevSer":"J. Phycol.","StandardTitleMon":null,"StartPage":326,"Pages":10,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Ciancia","Firstname":null,"Initials":"M.","Affiliation":"Univ Buenos Aires, Fac Agron,Dept Biol Aplicada & Alimentos CIHIDECAR CONICET, Catedra Quim Biomol,Buenos Aires, DF, Argentina.","Discriminator":null,"CorporateFlag":0,"BEntID":230228,"AutID":181126,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Alberghina","Firstname":null,"Initials":"J.","Affiliation":"Univ Buenos Aires, Fac Ciencias Exactas & Nat,Dept Ecol Genet & Evoluc, RA-1428 Buenos Aires, DF, Argentina.","Discriminator":null,"CorporateFlag":0,"BEntID":230228,"AutID":181127,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Arata","Firstname":null,"Initials":"P.X.","Affiliation":"Univ Buenos Aires, Fac Agron,Dept Biol Aplicada & Alimentos CIHIDECAR CONICET, Catedra Quim Biomol,Buenos Aires, DF, Argentina.","Discriminator":null,"CorporateFlag":0,"BEntID":230228,"AutID":181128,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Benavides","Firstname":null,"Initials":"H.","Affiliation":"Inst Nacl Invest & Desarrollo Pesquero INIDEP, BuenosAires, DF, Argentina.","Discriminator":null,"CorporateFlag":0,"BEntID":230228,"AutID":181129,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Leliaert","Firstname":"Frederik","Initials":"F.","Affiliation":"Phycology Research Group, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":230228,"AutID":133476,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biologie; Onderzoeksgroep Fycologie","ORCID":"0000-0002-4627-7318","PersID":227,"InsID":651},{"AutName":"Verbruggen","Firstname":"Heroen","Initials":"H.","Affiliation":"Phycology Research Group, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":230228,"AutID":133578,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biologie; Onderzoeksgroep Fycologie","ORCID":"0000-0002-6305-4749","PersID":3758,"InsID":651},{"AutName":"Estevez","Firstname":null,"Initials":"J.M.","Affiliation":"Univ Buenos Aires, Fac Ciencias Exactas & Nat,Inst Fisiol Biol Mol & Neurociencias IFIBYNE CONI, RA-1428 Buenos Aires,DF, Argentina.","Discriminator":null,"CorporateFlag":0,"BEntID":230228,"AutID":181132,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":279161,"LibID":36,"BRefID":238541,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"279161","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open Marien Archief","URL":null,"ClassifID":235,"Classification":"Open Marine Archive","ReqLink":null,"ClassifTypID":5,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":0,"InternalReq":0,"LoggedInReq":0,"Disclaimer":"Disclaimer_OMA","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Blackwell Science","City":"New York"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Bryopsis plumose"},{"TaxTerm":"Chlorophyta"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":230228,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":230228,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1111/j.1529-8817.2012.01131.x","externalID":"10.1111/j.1529-8817.2012.01131.x","URLTypeCode":"DOI","URLID":34147,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Bryopsis plumose","AphiaID":704053,"TaxtID":40528},{"TaxTerm":"Chlorophyta [green algae]","AphiaID":801,"TaxtID":15129}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Bryopsis plumose","DutchTerm":null,"AphiaID":704053,"TaxtID":40528},{"TaxTerm":"Chlorophyta","DutchTerm":"Groenwieren","AphiaID":801,"TaxtID":15129}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2014-05-18 21:53:46.440000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2015-10-29 08:40:10.340000","timezone_type":3,"timezone":"Europe/Brussels"}}}