{"refrec":{"BRefID":239354,"RR":"<b>Phuoc, L.H.; Corteel, M.; Thanh, N.C.; Nauwynck, H.J.; Pensaert, M.B.; Alday-Sanz, V.; Van Den Broeck, W.; Sorgeloos, P.; Bossier, P.</b> (2009). Effect of dose and challenge routes of <i>Vibrio</i> spp. on co-infection with white spot syndrome virus in <i>Penaeus vannamei</i>. <i>Aquaculture 290(1-2)</i>: 61-68. <a href=\"http://dx.doi.org/10.1016/j.aquaculture.2009.02.004\" target=\"_blank\">dx.doi.org/10.1016/j.aquaculture.2009.02.004</a>","BEntID":231039,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquaculture 290(1-2)</i>: 61-68. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2009.02.004\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2009.02.004</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Phuoc, L.H.; Corteel, M.; Thanh, N.C.; Nauwynck, H.J.; Pensaert, M.B.; Alday-Sanz, V.; Van Den Broeck, W.; Sorgeloos, P.; Bossier, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Phuoc, L.H. <i>et al.</i>","Englishabstract":"This study was conducted to investigate the effect of dose and challenge routes of <i>Vibrio</i> spp. on co-infection with white spot syndrome virus (WSSV) in specific pathogen-free (SPF) <i>Penaeus vannamei</i> shrimp. Juvenile shrimp were first injected with WSSV at a dose of 30 SID<sub>50</sub> shrimp<sup>- 1</sup> (SID<sub>50</sub> = shrimp infectious dose with 50% endpoint) and 24 h later with 10<sup>3</sup>, 10<sup>4</sup>, 10<sup>5</sup> or 10<sup>6</sup> CFU shrimp<sup>- 1</sup> of <i>V. campbellii</i>. Controls did not die during the experiment, except the ones that received 10<sup>6</sup> CFU shrimp<sup>- 1</sup> (35–65%). In WSSV-inoculated shrimp, the 100% cumulative mortality were reached at 144–360 h post injection (hpi). WSSV-infected shrimp died much faster when injected with at least 10<sup>4</sup> CFU of <i>V. campbellii</i> with the 100% cumulative mortality reached at 48–96 hpi of virus. The density of <i>V. campbellii</i> in haemolymph of co-infected moribund shrimp collected 6 h after <i>V. campbellii</i> injection was significantly higher than that in shrimp injected with <i>V. campbellii</i> only. There was no difference in the number of WSSV-infected cells between shrimp inoculated with WSSV only, compared to dually inoculated ones. Shrimp which were first injected with WSSV and 24 h (or 48 h) later exposed to 10<sup>6</sup>, 10<sup>7</sup>, or 10<sup>8</sup> CFU ml<sup>- 1</sup> of <i>V. campbellii</i> by immersion did not show any accelerated mortality. When WSSV-infected shrimp were challenged with another <i>Vibrio</i> species, <i>V. harveyi</i> BB120, no accelerated mortality was noted in WSSV-infected shrimp injected with 10<sup>6</sup> CFU shrimp<sup>- 1</sup> of <i>V. harveyi</i> BB120.In conclusion, it can be stated that the synergistic effect between WSSV and <i>Vibrio</i> is influenced by the dose, species and infection route of inoculation of the <i>Vibrio</i> bacteria.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Effect of dose and challenge routes of <i>Vibrio</i> spp. on co-infection with white spot syndrome virus in <i>Penaeus vannamei</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Co-infection; Challenge route; Dose; Shrimp; Vibrio; WSSV</td>","OtherDescriptors":null,"Notes":null,"AnaPub":2009,"MonPub":null,"DateUpdate":"2015-11-06","DateCreate":"2014-06-19","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000265705900009","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquaculture.2009.02.004"},"refs":null,"anarec":{"AnaID":239354,"PubliDate":2009,"Pagination":"61-68","XtraPublOfAnaID":null,"ISBN":null,"Volume":"290","Issue":"1-2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42190,"SerRR":"Aquaculture. 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ISSN 0044-8486; e-ISSN 1873-5622","StandardTitleSer":"Aquaculture","ISSN":"0044-8486","AbbrevSer":"Aquaculture","StandardTitleMon":null,"StartPage":61,"Pages":8,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Phuoc","Firstname":"Le Hong","Initials":"L.H.","Affiliation":"Laboratory of Aquaculture & Artemia Reference Center, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":231039,"AutID":83080,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":null,"PersID":12477,"InsID":22},{"AutName":"Corteel","Firstname":"Mathias","Initials":"M.","Affiliation":"Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":231039,"AutID":155587,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; 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