{"refrec":{"BRefID":24268,"RR":"<b>Backeljau, T.; Frias Martins, A.M.; Gosling, E.; Grahame, J.; Mill, P.; Brito, C.; Clarke, A.; Medeiros, R.; Small, M.; Wilding, C.; Wilson, I.; Winnepenninckx, B.; Clarke, R.; De Wolf, H.</b> (2001). Periwinkles (Gastropoda, Littorinidae) as a model for studying patterns and dynamics of marine biodiversity. <i>Bull. Kon. Belg. Inst. Natuurwet. Biologie 71(Suppl.)</i>: 43-65","BEntID":24268,"PublicFlag":1,"CheckedFlag":0,"wosflag":null,"vabbflag":null,"RefStringPartII":". <i>Bull. Kon. Belg. Inst. Natuurwet. Biologie 71(Suppl.)</i>: 43-65","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Backeljau, T.; Frias Martins, A.M.; Gosling, E.; Grahame, J.; Mill, P.; Brito, C.; Clarke, A.; Medeiros, R.; Small, M.; Wilding, C.; Wilson, I.; Winnepenninckx, B.; Clarke, R.; De Wolf, H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Backeljau, T. <i>et al.</i>","Englishabstract":"This paper presents a summary synthesis of an international EC- funded research program in which periwinkles are used as a model group to study patterns and dynamics in marine biodiversity. In the first part of the paper, a number of techniques and markers to assess biodiversity in periwinkles are illustrated and evaluated, while protocols for DNA-extraction, Single-Strand Conformation Polymorphisms (SSCP) and PCR-amplification of a number of nuclear and mitochondrial DNA markers are provided. The second part of the paper presents a brief overview of the application of these techniques in the analysis of genetic diversity in two periwinkle taxa with contrasting developmental modes, viz. <i>Littorifla striata</i> and the <i>L. saxatilis</i> complex. Finally, these results are dis- cussed in the light of the implementation of the Evolutionarily significant Unit (ESU) and Management Unit (MU) concepts in (marine) conservation biology. It is concluded that, although these operational concepts are more appropriate than species level systematics for delineating relevant intraspecific diversity, they still may not adequately represent non-molecular evolutionarily relevant, diversity.","AbstractOtherLang":"Cet article présente une brève synthèse d'un projet international financié par la CE, dans lequel les littorines sont choisies comme groupe modèle pour l'étude de la structure et la dynamique de la biodiversité marine. Dans la première partie de l'article, on présente une illustration et une évaluation d'un nombre de techniques et de marqueurs moléculaires utilisés dans l'étude de la biodiversité des littorines. De plus on propose des protocoles pour l'extraction d' ADN, pour d'analyses de \"Single-Strand Conformation Polymorphisms (SSCP)\" et pour l'amplification par PCR d'un nombre de marqueurs d' ADN nucléaires et mitochondriaux. La deuxième partie de I' article donne un bref aperçu de l' application de ces techniques dans l'analyse de la diversité génétique dans deux taxons de littorines avec des modes de développement alternatifs: <i>Littorina striata</i> et le complexe de <i>L. saxatilis</i>. Finalement, les résultats de ces analyses sont discutés dans le cadre de l'application des conceptes comme les \"Evolutionarily Significant Units (ESU)\" et les \"Management Units (MU)\" dans la biologie de conservation (marine). Bien que ces \"ESUs\" et \"MUs\" soient meilleurs que la systématique au niveau d'espèces, pour délimiter la diversité intraspecifique, ils ne représentent pas suffisamment la diversité non-moléculaire pertinente du point de vue d'evolution.","BibLvlCode":"AS","StandardTitle":"Periwinkles (Gastropoda, Littorinidae) as a model for studying patterns and dynamics of marine biodiversity","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2001,"MonPub":null,"DateUpdate":"2003-07-15","DateCreate":"2002-04-24","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":24268,"PubliDate":2001,"Pagination":"43-65","XtraPublOfAnaID":null,"ISBN":null,"Volume":"71","Issue":"Suppl.","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":44202,"SerRR":"Bulletin van het Koninklijk Belgisch Instituut voor Natuurwetenschappen. 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