{"refrec":{"BRefID":24623,"RR":"<b>De Boeck, M.; Kirsch-Volders, M.</b> (1997). <i>Nereis virens</i> (Annelida: Polychaeta) is not an adequate sentinel species to assess the genotoxic risk (comet assay) of PAH exposure to the environment. <i>Environ. Mol. Mutagen. 30(1)</i>: 82-90. <a href=\"http://dx.doi.org/10.1002/(SICI)1098-2280(1997)30:1<82::AID-EM11>3.0.CO;2-M\" target=\"_blank\">http://dx.doi.org/10.1002/(SICI)1098-2280(1997)30:1<82::AID-EM11>3.0.CO;2-M</a>","BEntID":24623,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Environ. Mol. Mutagen. 30(1)</i>: 82-90. <a href=\"http://dx.doi.org/10.1002/(SICI)1098-2280(1997)30:1<82::AID-EM11>3.0.CO;2-M\" target=\"_blank\">http://dx.doi.org/10.1002/(SICI)1098-2280(1997)30:1<82::AID-EM11>3.0.CO;2-M</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"De Boeck, M.; Kirsch-Volders, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"De Boeck, M.; Kirsch-Volders, M.","Englishabstract":"Polychaetes, because of their bioturbation capacity, play an important role in the distribution of anthropogenic contaminants (including polycyclic aromatic hydrocarbons [PAHs]) throughout the sediments. In this work the use of Nereis virens (Annelida: Polychaeta) as a bioindicator to assess the genotoxic risk of PAH exposure for the environment was evaluated. For this purpose the alkaline single cell gel electrophoresis [comet] assay was applied on the coelomocytes of in vivo exposed Nereis virens. Benzo[a]pyrene (B[a]P) was chosen because it is classified by the IARC (International Agency for Research on Cancer) as \"probably carcinogenic to humans\" and because its mechanisms of action are well-known. Nereis virens was exposed to B[a]P in concentrations of 0.3, 0.6, 10, 20, 35 and 45 mg/ml by an intracoelomic injection of B[a]P (20 microliters) dissolved in dimethyl sulphoxide (DMSO). A solvent control with DMSO, a positive control with ethyl methane sulphonate (EMS) (12.1 mg/ml) and a negative control were included in each experiment. For each treatment four animals were analysed. After 1 hr treatment coelomocytes were harvested by puncturing the coelomic cavity with a sharpened Pasteur pipette, mixed with 0.5% low melting point agarose and sandwiched between two other gel layers. Ethidium bromide stained nuclei were analysed for tail length and tail moment. 12.1 mg/ml EMS, pure DMSO (98.9%) and B[a]P in all tested concentrations induced a statistically significant increase of DNA single strand breaks in the comet assay. The effect of B[a]P, however, was only at the highest concentration (45 mg/ml) significantly stronger than the effect of DMSO alone. Although a relatively large heterogeneity in the results could be observed, these experiments clearly showed that Nereis virens is not suited as a sentinel species for the assessment of the genotoxic risk of PAH exposure because this species seems to be very resistant to benzo[a]pyrene.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"<i>Nereis virens</i> (Annelida: Polychaeta) is not an adequate sentinel species to assess the genotoxic risk (comet assay) of PAH exposure to the environment","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-06 01:31:58.743573","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"comet assay; coelomocytes; DNA damage; benzo[a]pyrene; Nereis virens; bioindicator","OtherDescriptors":"comet assay; coelomocytes; DNA damage; benzopyrene; <i>Nereis virens</i>; bioindicator","Notes":null,"AnaPub":1997,"MonPub":null,"DateUpdate":"2016-10-09","DateCreate":"2002-05-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1997XP50900011","VABBcode":null,"OpenAcc":0,"DOI":"10.1002/(SICI)1098-2280(1997)30:1<82::AID-EM11>3.0.CO;2-M"},"refs":null,"anarec":{"AnaID":24623,"PubliDate":1997,"Pagination":"82-90","XtraPublOfAnaID":null,"ISBN":null,"Volume":"30","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45605,"SerRR":"Environmental and Molecular Mutagenesis. Liss: New York, N.Y..  ISSN 0893-6692; e-ISSN 1098-2280","StandardTitleSer":"Environmental and Molecular Mutagenesis","ISSN":"0893-6692","AbbrevSer":"Environ. Mol. 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