{"refrec":{"BRefID":246908,"RR":"<b>Li, W.; Van Thao, N.; Corteel, M.; Dantas-Lima, J.J.; Thuong, K.V.; Tuan, V.V.; Bossier, P.; Sorgeloos, P.; Nauwynck, H.</b> (2014). Characterization of a primary cell culture from lymphoid organ of <i>Litopenaeus vannamei</i> and use for studies on WSSV replication. <i>Aquaculture 433</i>: 157-163. <a href=\"http://dx.doi.org/10.1016/j.aquaculture.2014.05.046\" target=\"_blank\">dx.doi.org/10.1016/j.aquaculture.2014.05.046</a>","BEntID":238608,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquaculture 433</i>: 157-163. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2014.05.046\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2014.05.046</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Li, W.; Van Thao, N.; Corteel, M.; Dantas-Lima, J.J.; Thuong, K.V.; Tuan, V.V.; Bossier, P.; Sorgeloos, P.; Nauwynck, H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Li, W. <i>et al.</i>","Englishabstract":"Shrimp aquaculture is a booming agro-industry worldwide. Due to intensification of shrimp farming, pathogens emerge. Control of these pathogens especially viral pathogens is essential for a further expansion of this industry. Until now, the lack of shrimp cell lines has limited research on shrimp viral pathogens. In this context, a primary culture from the lymphoid organ of <i>Litopenaeus vannamei</i> was developed and standardized as a platform for further research on white spot syndrome virus (WSSV). Explants from the lymphoid organ of <i>L. vannamei</i> were cultured in 2 × L-15 (Leibovitz-15) medium supplemented with 20% fetal bovine serum (FBS), 10% Chen's salt, penicillin (100 IU/ml) & streptomycin (100 µg/ml), gentamicin (50 µg/ml) and fungizone (0.25 µg/ml) with a pH of 7.5. Gelatin (0.1%)-coated culture plates promoted the migration of cells from the explants and cell survival. 600 µg/ml cholesterol and 1000 µg/ml l-glutathione (GSH) both enhanced cell survival and performance <i>in vitro</i>. Susceptibility of lymphoid organ cells for infection with white spot syndrome virus (WSSV) was determined by indirect immunofluorescence (IIF) staining by monoclonal antibodies against VP28 (W29) and goat anti-mouse IgG-FITC. FITC positive signals in the nuclei starting from 9 h post inoculation (hpi) demonstrated the susceptibility of the cells for WSSV infection in these cultures. This cell culture system will be used in the future as a tool for studying host–virus interactions in WSSV pathogenesis.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Characterization of a primary cell culture from lymphoid organ of <i>Litopenaeus vannamei</i> and use for studies on WSSV replication","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"L. vannamei; Lymphoid organ; Cell culture; Cholesterol; L-Glutathione;White spot syndrome virus (WSSV)","OtherDescriptors":null,"Notes":null,"AnaPub":2014,"MonPub":null,"DateUpdate":"2015-10-28","DateCreate":"2015-05-21","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000342529400024","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquaculture.2014.05.046"},"refs":null,"anarec":{"AnaID":246908,"PubliDate":2014,"Pagination":"157-163","XtraPublOfAnaID":null,"ISBN":null,"Volume":"433","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42190,"SerRR":"Aquaculture. 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