{"refrec":{"BRefID":246942,"RR":"<b>Bigot, L; Beets, I.; Dubos, P; Boudry, P; Schoofs, L.; Favrel, P</b> (2014). Functional characterization of a short neuropeptide F-related receptor in a lophotrochozoan, the mollusk <i>Crassostrea gigas</i>. <i>J. Exp. Biol. 217(16)</i>: 2974-2982. <a href=\"http://dx.doi.org/10.1242/jeb.104067\" target=\"_blank\">dx.doi.org/10.1242/jeb.104067</a>","BEntID":238642,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Exp. Biol. 217(16)</i>: 2974-2982. <a href=\"https://dx.doi.org/10.1242/jeb.104067\" target=\"_blank\">https://dx.doi.org/10.1242/jeb.104067</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Bigot, L; Beets, I.; Dubos, P; Boudry, P; Schoofs, L.; Favrel, P","OrigTitleTranslFlag":0,"Authorstringtrunc":"Bigot, L <i>et al.</i>","Englishabstract":"Members of the short neuropeptide F (sNPF) family of peptides and their cognate receptors play key roles in a variety of physiological processes in arthropods. <i>In silico</i> screening of GigasDatabase, a specific expressed sequence tag database from the Pacific oyster <i>Crassostrea gigas</i>, resulted in the identification of a receptor (<i>Cg</i>-sNPFR-like) phylogenetically closely related to sNPF receptors (sNPFRs) of insects. A reverse endocrinology approach was undertaken to identify the peptide ligand(s) of this orphan receptor. Though structurally distinct from insect sNPFs, three RFamide peptides derived from the same precursor, i.e. GSLFRFamide, SSLFRFamide and GALFRFamide, specifically activate the receptor in a dose-dependent manner, with respective EC<sub>50</sub> values (half-maximal effective concentrations) of 1.1, 2.1 and 4.1 µmol l<sup>-1</sup>. We found that both <i>Cg</i>-sNPFR-like receptor and LFRFamide encoding transcripts are expressed in the oyster central nervous system and in other tissues as well, albeit at lower levels. Mass spectrometry analysis confirmed the wide distribution of LFRFamide mature peptides in several central and peripheral tissues. The <i>Cg</i>-sNPFR-like receptor was more abundantly expressed in ganglia of females than of males, and upregulated in starved oysters. In the gonad area, highest receptor gene expression occurred at the start of gametogenesis, when storage activity is maximal. Our results suggest that signaling of LFRFamide peptides through the <i>Cg</i>-sNPFR-like receptor might play a role in the coordination of nutrition, energy storage and metabolism in <i>C. gigas</i>, possibly by promoting storage at the expense of reproduction.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Functional characterization of a short neuropeptide F-related receptor in a lophotrochozoan, the mollusk <i>Crassostrea gigas</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-18 01:31:41.408683","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Oyster; Crassostrea gigas; sNPF; Energy metabolism; Reproduction;Starvation","OtherDescriptors":null,"Notes":null,"AnaPub":2014,"MonPub":null,"DateUpdate":"2015-10-28","DateCreate":"2015-05-21","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000341190300024","VABBcode":null,"OpenAcc":0,"DOI":"10.1242/jeb.104067"},"refs":null,"anarec":{"AnaID":246942,"PubliDate":2014,"Pagination":"2974-2982","XtraPublOfAnaID":null,"ISBN":null,"Volume":"217","Issue":"16","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43227,"SerRR":"The Journal of Experimental Biology. Cambridge University Press: London.  ISSN 0022-0949; e-ISSN 1477-9145","StandardTitleSer":"The Journal of Experimental Biology","ISSN":"0022-0949","AbbrevSer":"J. Exp. Biol.","StandardTitleMon":null,"StartPage":2974,"Pages":9,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Bigot","Firstname":null,"Initials":"L","Affiliation":"Univ Caen Basse Normandie, Biol Organismes Aquat & Ecosyst BOREA, F-14032 Caen 05, France.","Discriminator":null,"CorporateFlag":0,"BEntID":238642,"AutID":195762,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Beets","Firstname":"Isabel","Initials":"I.","Affiliation":"Department of Biology, Functional Genomics and Proteomics Group, KU Leuven","Discriminator":null,"CorporateFlag":0,"BEntID":238642,"AutID":195764,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Katholieke Universiteit Leuven; Departement Biologie; Laboratorium voor Ontwikkelingsfysiologie, Genomics en Proteomics","ORCID":"0000-0002-0968-4360","PersID":31371,"InsID":495},{"AutName":"Dubos","Firstname":"M","Initials":"P","Affiliation":"Univ Caen Basse Normandie, Biol Organismes Aquat & Ecosyst BOREA, F-14032 Caen 05, France.","Discriminator":null,"CorporateFlag":0,"BEntID":238642,"AutID":195765,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Boudry","Firstname":null,"Initials":"P","Affiliation":"IFREMER, UMR 6539, LEMAR, Unite Physiol Fonct Organismes Marins, F-29280 Plouzane, France.","Discriminator":null,"CorporateFlag":0,"BEntID":238642,"AutID":195767,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Schoofs","Firstname":"Liliane","Initials":"L.","Affiliation":"Department of Biology, Functional Genomics and Proteomics Group, KU Leuven","Discriminator":null,"CorporateFlag":0,"BEntID":238642,"AutID":195768,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Katholieke Universiteit Leuven; Departement Biologie; Laboratorium voor Ontwikkelingsfysiologie, Genomics en Proteomics","ORCID":"0000-0002-1673-432X","PersID":3543,"InsID":495},{"AutName":"Favrel","Firstname":null,"Initials":"P","Affiliation":"Univ Caen Basse Normandie, Biol Organismes Aquat & Ecosyst BOREA, F-14032 Caen 05, France.","Discriminator":null,"CorporateFlag":0,"BEntID":238642,"AutID":195769,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":278994,"LibID":36,"BRefID":246942,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"278994","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open Marien Archief","URL":null,"ClassifID":235,"Classification":"Open Marine Archive","ReqLink":null,"ClassifTypID":5,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":0,"InternalReq":0,"LoggedInReq":0,"Disclaimer":"Disclaimer_OMA","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Cambridge University Press","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Crassostrea gigas"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":238642,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":238642,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1242/jeb.104067","externalID":"10.1242/jeb.104067","URLTypeCode":"DOI","URLID":39872,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Crassostrea gigas [Portuguese oyster]","AphiaID":836033,"TaxtID":22937}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Crassostrea gigas","DutchTerm":"Japanse oester","AphiaID":836033,"TaxtID":22937}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2015-05-21 21:47:51.050000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2015-10-28 08:22:32.133000","timezone_type":3,"timezone":"Europe/Brussels"}}}