{"refrec":{"BRefID":246953,"RR":"<b>Stewart, P; Deschamps, H; Witten, P.E.; Le Luyer, J; Proulx, E; Huysseune, A.; Bureau, P; Vandenberg, W</b> (2014). X-ray-based morphometrics: an approach to diagnose vertebral abnormalities in under-mineralized vertebrae of juvenile triploid all-female rainbow trout (<i>Oncorhynchus mykiss</i>) fed with a phosphorus deficient diet. <i>J. Appl. Ichthyol. 30(4)</i>: 796-803. <a href=\"http://dx.doi.org/10.1111/jai.12520\" target=\"_blank\">dx.doi.org/10.1111/jai.12520</a>","BEntID":238653,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>J. Appl. Ichthyol. 30(4)</i>: 796-803. <a href=\"https://dx.doi.org/10.1111/jai.12520\" target=\"_blank\">https://dx.doi.org/10.1111/jai.12520</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":1,"BrackishFlag":1,"TerrestrialFlag":0,"Authorstring":"Stewart, P; Deschamps, H; Witten, P.E.; Le Luyer, J; Proulx, E; Huysseune, A.; Bureau, P; Vandenberg, W","OrigTitleTranslFlag":0,"Authorstringtrunc":"Stewart, P <i>et al.</i>","Englishabstract":"Extended phosphorus (P) deficiency has been linked to an increase of skeletal deformities in farmed salmonids. To date, X-ray examination still is the most practical way to detect abnormalities that are not externally visible. The aim of this study was to develop a standardized method based on empirical measurements that provides an early radiograph-based detection of deformities. Triploid all-female rainbow trout (60.8 ± 1.6 g) were fed with phosphorus (P)-deficient and P-sufficient diets (total P = 0.5% and 0.92%, respectively). Experiments were carried out in 12 circular re-circulating tanks (n = 140/tank; 8.5–52.7 kg m<sup>-3</sup>; 12 ± 0.3°C; 18L: 6D). All fish were x-rayed at week 5, 15 and 24 to monitor vertebral abnormalities. Fish were assigned to a vertebral phenotype (normal, biconcave or compressed) when all vertebrae of the caudal region (V31-44) showed the same type of abnormalities. At week 9, 18 and 27, ten fish per tank were randomly sampled according to their vertebral phenotype. Vertebrae V38–V39 were collected and a second X-ray was performed to confirm the above-mentioned phenotypes. Morphometrics (vertebral length and height; intervertebral distances and vertebral body angles) were measured directly on radiographs. For all parameters measured, comparisons were made between normal P-sufficient fish and different scenarios of vertebral development (normal, restored, stable and aggravated) in P-deficient fish. The two parameters showing the most sensitivity were the median intervertebral distance and dorsal/ventral vertebral endplate angles. When analysed together, these two parameters allow a quantitative distinction of the different vertebral phenotypes. Measuring dorsal/ventral vertebral endplate angles seems a promising approach to detect aberrant vertebral development at early stages as slight changes were observed in P-deficient vertebrae that were assessed as normal by visual examination. Further studies including histological examinations and comparison with other radiological imaging techniques are required to confirm these results.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"X-ray-based morphometrics: an approach to diagnose vertebral abnormalities in under-mineralized vertebrae of juvenile triploid all-female rainbow trout (<i>Oncorhynchus mykiss</i>) fed with a phosphorus deficient diet","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2014,"MonPub":null,"DateUpdate":"2015-10-28","DateCreate":"2015-05-21","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000340243200027","VABBcode":null,"OpenAcc":0,"DOI":"10.1111/jai.12520"},"refs":null,"anarec":{"AnaID":246953,"PubliDate":2014,"Pagination":"796-803","XtraPublOfAnaID":null,"ISBN":null,"Volume":"30","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43023,"SerRR":"Journal of Applied Ichthyology = Zeitschrift für angewandte Ichthyologie. 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