{"refrec":{"BRefID":248410,"RR":"<b>Boedeker, C.; Karsten, U.; Leliaert, F.; Zuccarello, G.C.</b> (2013). Molecular, biochemical and morphological data suggest an affiliation of <i>Spongiochrysis hawaiiensis</i> with the Trentepohliales (Ulvophyceae, Chlorophyta). <i>Phycol. Res. 61(2)</i>: 133-144. <a href=\"http://dx.doi.org/10.1111/pre.12011\" target=\"_blank\">http://dx.doi.org/10.1111/pre.12011</a>","BEntID":240115,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Phycol. Res. 61(2)</i>: 133-144. <a href=\"http://dx.doi.org/10.1111/pre.12011\" target=\"_blank\">http://dx.doi.org/10.1111/pre.12011</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Boedeker, C.; Karsten, U.; Leliaert, F.; Zuccarello, G.C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Boedeker, C. <i>et al.</i>","Englishabstract":"The aeroterrestrial, unicellular green alga <em>Spongiochrysis hawaiiensis</em> had been included in the ulvophycean order Cladophorales based on small subunit (SSU) rDNA sequence data, and represents so far the only fully terrestrial member of this order. Other characteristics of <em>S.?hawaiiensis</em> that are atypical for Cladophorales include the presence of large amounts of carotenoids and a budding-like mode of cell division. As the position of this terrestrial, unicellular alga in an order of aquatic, multicellular green algae is unusual, we re-evaluated the phylogenetic relationships of this enigmatic organism based on supplementary SSU rDNA sequences as well as novel large ribosomal subunit (LSU) rDNA and internal transcribed spacer (ITS rDNA) sequences. Additionally, we examined several morphological characters of <em>S.?hawaiiensis</em>, as well as low molecular weight carbohydrate (LMWC) patterns of <em>S.?hawaiiensis</em> and members of the Cladophorales and Trentepohliales as potential chemotaxonomic markers. We found <em>S.?hawaiiensis</em> to be uninucleate. The analysis of the LMWC content detected the presence of the polyol erythritol in <em>S.?hawaiiensis</em> and in the Trentepohliales, while this compound was missing in the Cladophorales. The phylogenetic analyses of the novel sequences placed <em>S.?hawaiiensis</em> in the terrestrial Trentepohliales. 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