{"refrec":{"BRefID":250538,"RR":"<b>Collard, F.; Gilbert, B.; Eppe, G.; Parmentier, E.; Das, K.</b> (2015). Detection of anthropogenic particles in fish stomachs: An isolation method adapted to identification by Raman spectroscopy. <i>Arch. Environ. Contam. Toxicol. 69(3)</i>: 331-339. <a href=\"https://dx.doi.org/10.1007/s00244-015-0221-0\" target=\"_blank\">https://dx.doi.org/10.1007/s00244-015-0221-0</a>","BEntID":242232,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Arch. Environ. Contam. Toxicol. 69(3)</i>: 331-339. <a href=\"https://dx.doi.org/10.1007/s00244-015-0221-0\" target=\"_blank\">https://dx.doi.org/10.1007/s00244-015-0221-0</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Collard, F.; Gilbert, B.; Eppe, G.; Parmentier, E.; Das, K.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Collard, F. <i>et al.</i>","Englishabstract":"Microplastic particles (MP) contaminate oceans and affect marine organisms in several ways. Ingestion combined with food intake is generally reported. However, data interpretation often is circumvented by the difficulty to separate MP from bulk samples. Visual examination often is used as one or the only step to sort these particles. However, color, size, and shape are insufficient and often unreliable criteria. We present an extraction method based on hypochlorite digestion and isolation of MP from the membrane by sonication. The protocol is especially well adapted to a subsequent analysis by Raman spectroscopy. The method avoids fluorescence problems, allowing better identification of anthropogenic particles (AP) from stomach contents of fish by Raman spectroscopy. It was developed with commercial samples of microplastics and cotton along with stomach contents from three different Clupeiformes fishes: Clupea harengus, Sardina pilchardus, and Engraulis encrasicolus. The optimized digestion and isolation protocol showed no visible impact on microplastics and cotton particles while the Raman spectroscopic spectrum allowed the precise identification of microplastics and textile fibers. Thirty-five particles were isolated from nine fish stomach contents. Raman analysis has confirmed 11 microplastics and 13 fibers mainly made of cellulose or lignin. Some particles were not completely identified but contained artificial dyes. The novel approach developed in this manuscript should help to assess the presence, quantity, and composition of AP in planktivorous fish stomachs.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Detection of anthropogenic particles in fish stomachs: An isolation method adapted to identification by Raman spectroscopy","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-29 01:31:58.085532","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2015,"MonPub":null,"DateUpdate":"2017-08-04","DateCreate":"2015-10-15","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000361493800008","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s00244-015-0221-0"},"refs":null,"anarec":{"AnaID":250538,"PubliDate":2015,"Pagination":"331-339","XtraPublOfAnaID":null,"ISBN":null,"Volume":"69","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45321,"SerRR":"Archives of Environmental Contamination and Toxicology. Springer: New York.  ISSN 0090-4341; e-ISSN 1432-0703","StandardTitleSer":"Archives of Environmental Contamination and Toxicology","ISSN":"0090-4341","AbbrevSer":"Arch. Environ. Contam. Toxicol.","StandardTitleMon":null,"StartPage":331,"Pages":9,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Collard","Firstname":"France","Initials":"F.","Affiliation":"Functional and Evolutionary Morphology Laboratory University of Liège","Discriminator":null,"CorporateFlag":0,"BEntID":242232,"AutID":175585,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Université de Liège; Faculté des Sciences; Département de Biologie, Ecologie et Evolution; Laboratoire de Morphologie Fonctionelle et Evolutive","ORCID":null,"PersID":29500,"InsID":3836},{"AutName":"Gilbert","Firstname":"Bernard","Initials":"B.","Affiliation":"Inorganic Analytical Chemistry Laboratory, Department of Chemistry, University of Liège","Discriminator":null,"CorporateFlag":0,"BEntID":242232,"AutID":201404,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MSLAB","InsFSN":"Université de Liège; Faculté des Sciences; Département de Chimie; Laboratoire de Spectrométrie de Masse","ORCID":null,"PersID":470,"InsID":1905},{"AutName":"Eppe","Firstname":"Gauthier","Initials":"G.","Affiliation":"Inorganic Analytical Chemistry Laboratory, Department of Chemistry, University of Liège","Discriminator":null,"CorporateFlag":0,"BEntID":242232,"AutID":263266,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Université de Liège; Faculté des Sciences; Département de Chimie","ORCID":null,"PersID":4691,"InsID":9590},{"AutName":"Parmentier","Firstname":"Eric","Initials":"E.","Affiliation":"Functional and Evolutionary Morphology Laboratory University of Liège","Discriminator":null,"CorporateFlag":0,"BEntID":242232,"AutID":175586,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Université de Liège; Faculté des Sciences; Département de Biologie, Ecologie et Evolution; Laboratoire de Morphologie Fonctionelle et Evolutive","ORCID":"0000-0002-0391-7530","PersID":7071,"InsID":3836},{"AutName":"Das","Firstname":"Krishna","Initials":"K.","Affiliation":"Laboratory for Oceanology, MARE Centre, University of Liege","Discriminator":null,"CorporateFlag":0,"BEntID":242232,"AutID":180917,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Université de Liège; Faculté des Sciences; Département de Biologie, Ecologie et Evolution; Laboratoire d'Océanologie Biologique","ORCID":"0000-0003-2689-3348","PersID":3862,"InsID":459}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":278459,"LibID":36,"BRefID":250538,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"278459","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open Marien Archief","URL":null,"ClassifID":235,"Classification":"Open Marine Archive","ReqLink":null,"ClassifTypID":5,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":0,"InternalReq":0,"LoggedInReq":0,"Disclaimer":"Disclaimer_OMA","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Springer","City":"New York"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":242232,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":242232,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1007/s00244-015-0221-0","externalID":"10.1007/s00244-015-0221-0","URLTypeCode":"DOI","URLID":56620,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Haspeslagh, Jan, J.","newSesDate":{"date":"2015-10-15 15:02:50.967000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2017-08-04 08:22:33.740000","timezone_type":3,"timezone":"Europe/Brussels"}}}