Document of bibliographic reference 251876
BibliographicReference record
- Type
- Bibliographic resource
- Type of document
- Journal article
- BibLvlCode
- AS
- Title
- Spliced leader RNA trans-splicing discovered in copepods
- Abstract
- Copepods are one of the most abundant metazoans in the marine ecosystem, constituting a critical link in aquatic food webs and contributing significantly to the global carbon budget, yet molecular mechanisms of their gene expression are not well understood. Here we report the detection of spliced leader (SL) trans-splicing in calanoid copepods. We have examined nine species of wild-caught copepods from Jiaozhou Bay, China that represent the major families of the calanoids. All these species contained a common 46-nt SL (CopepodSL). We further determined the size of CopepodSL precursor RNA (slRNA; 108-158 nt) through genomic analysis and 3'-RACE technique, which was confirmed by RNA blot analysis. Structure modeling showed that the copepod slRNA folded into typical slRNA secondary structures. Using a CopepodSL-based primer set, we selectively enriched and sequenced copepod full-length cDNAs, which led to the characterization of copepod transcripts and the cataloging of the complete set of 79 eukaryotic cytoplasmic ribosomal proteins (cRPs) for a single copepod species. We uncovered the SL trans-splicing in copepod natural populations, and demonstrated that CopepodSL was a sensitive and specific tool for copepod transcriptomic studies at both the individual and population levels and that it would be useful for metatranscriptomic analysis of copepods.
- WebOfScience code
- https://www.webofscience.com/wos/woscc/full-record/WOS:000365487700001
- Bibliographic citation
- Yang, F.; Xu, D.; Zhuang, Y.; Yi, X.; Huang, Y.; Chen, H.; Lin, S.; Campbell, D.A.; Sturm, N.R.; Liu, G.; Zhang, H. (2015). Spliced leader RNA trans-splicing discovered in copepods. NPG Scientific Reports 5(17411): 10 pp. http://dx.doi.org/10.1038/srep17411
- Topic
- Marine
- Is peer reviewed
- true
- Access rights
- open access
- Is accessible for free
- true
Authors
- author
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- Name
- Feifei Yang
- author
-
- Name
- Donghui Xu
- author
-
- Name
- Yunyun Zhuang
- author
-
- Name
- Xiaoyan Yi
- author
-
- Name
- Yousong Huang
- author
-
- Name
- Hongjun Chen
- author
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- Name
- Senjie Lin
- author
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- Name
- David Campbell
- author
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- Name
- Nancy Sturm
- author
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- Name
- Guangxing Liu
- author
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- Name
- Huan Zhang