{"refrec":{"BRefID":251993,"RR":"<b>Tadonléké, R.D.; Pollet, T.; van Rijswijk, P.; Leberre, B.; Middelburg, J.J.</b> (2016). Effects of oxygen loss on carbon processing and heterotrophic prokaryotes from an estuarine ecosystem: results from stable isotope probing and cytometry analyses. <i>Est. Coast. 39(4)</i>: 992-1005. <a href=\"http://dx.doi.org/10.1007/s12237-015-0053-1\" target=\"_blank\">http://dx.doi.org/10.1007/s12237-015-0053-1</a>","BEntID":243690,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Est. Coast. 39(4)</i>: 992-1005. <a href=\"https://dx.doi.org/10.1007/s12237-015-0053-1\" target=\"_blank\">https://dx.doi.org/10.1007/s12237-015-0053-1</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tadonléké, R.D.; Pollet, T.; van Rijswijk, P.; Leberre, B.; Middelburg, J.J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tadonléké, R.D. <i>et al.</i>","Englishabstract":"Many aquatic ecosystems are experiencing a decline in their oxygen (O<sub>2</sub>) content and this is predicted to continue. Implications of this change on several properties of bacterioplankton (heterotrophic prokaryotes) remain however are poorly known. In this study, oxic samples (~170 µM O<sub>2</sub>?=?controls) from an oligohaline region of the Scheldt Estuary were purged with N<sub>2</sub> to yield low-O<sub>2</sub> samples (~69 µM O<sub>2</sub>?=?treatments); all were amended with <sup>13</sup>C-glucose and incubated in dark to examine carbon incorporation and cell size of heterotrophic prokaryotes, and relationships between organic matter (OM) degradation and phosphate (P) availability in waters following O<sub>2</sub> loss. Stable isotope (<sup>13</sup>C) probing of phospholipid fatty acids (PLFA) and flow cytometry were used. In samples that have experienced O<sub>2</sub> loss, PLFA biomass became higher, prokaryotic cells had significantly larger size and higher nucleic acid content, but P concentrations was lower, compared to controls. P concentration and OM degradation were positively related in controls, but uncoupled in low-O<sub>2</sub> samples. Moreover, the dominant PLFA 16:1<i>?</i>7c (likely mainly from Gram-negative bacteria) and the nucleic acid content of heterotrophic prokaryotic cells in low-O<sub>2</sub> samples explained (62–72 %) differences between controls and low-O<sub>2</sub> samples in P amounts. Shortly after incubations began, low-O<sub>2</sub> samples had consistently lower bacterial PLFA <sup>13</sup>C-enrichments, suggesting involvement of facultatively anaerobic metabolism in carbon incorporation, and supporting the view that this metabolic pathway is widespread among pelagic bacteria in coastal nutrient-rich ecosystems. Estimates based on <sup>13</sup>C-enrichment of PLFAs indicated that grazing by protozoa on some bacteria was stronger in low-O<sub>2</sub> samples than in controls, suggesting that the grazing pressure on some heterotrophic prokaryotes may increase at the onset of O<sub>2</sub> deficiency in nutrient-rich aquatic systems. These findings also suggest that physiological responses of heterotrophic prokaryotes to O<sub>2</sub> loss in such ecosystems include increases in cell activity, high carbon incorporation, and possibly phosphorus retention by cells that may contribute to reduce phosphate availability in waters.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Effects of oxygen loss on carbon processing and heterotrophic prokaryotes from an estuarine ecosystem: results from stable isotope probing and cytometry analyses","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Oxygen decline; Carbon processing; Heterotrophic prokaryotes; Phosphorus availability; Phospholipid fatty acids; Stable isotope probing; Flow cytometry","OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2016-09-07","DateCreate":"2015-12-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000377798600008","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s12237-015-0053-1"},"refs":null,"anarec":{"AnaID":251993,"PubliDate":2016,"Pagination":"992-1005","XtraPublOfAnaID":null,"ISBN":null,"Volume":"39","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":101945,"SerRR":"Estuaries and Coasts. 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