{"refrec":{"BRefID":252202,"RR":"<b>coluccia, E.; Deiana, A.M.; Cannas, R.; Salvadori, S.</b> (2006). Study of the nucleolar organizer regions in <i>Palinurus elephas</i> (Crustacea: Decapoda), <b><i>in</i></b>: Thessalou-Legaki, M. (Ed.) <i>Issues of decapod crustacean biology. Developments in Hydrobiology,</i> 184: pp. 75-82","BEntID":243898,"PublicFlag":1,"CheckedFlag":0,"wosflag":null,"vabbflag":null,"RefStringPartII":", <b><i>in</i></b>: Thessalou-Legaki, M. (Ed.) <i>Issues of decapod crustacean biology. Developments in Hydrobiology,</i> 184: pp. 75-82","DocTypID":17,"DocType":"Book chapters","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"coluccia, E.; Deiana, A.M.; Cannas, R.; Salvadori, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"coluccia, E. <i>et al.</i>","Englishabstract":"Using fluorescence in situ hybridization (FISH), chromomycin (CMA<sub>3</sub>) staining and silver staining, we studied the nucleolar organizer regions in the spiny lobster <i>Palinurus elephas</i> in order to extend our knowledge on the karyology of this commercially important species. Multiple NORs have been detected by FISH, and CMA<sub>3</sub> showed a good correspondence between the localization of GC-rich heterochromatin and the ribosomal genes mapped by FISH. In contrast, the number of Ag-positive regions was higher than the number of FISH and CMA<sub>3</sub> signals, which may be explained by silver staining of the kinetochores. 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