{"refrec":{"BRefID":255366,"RR":"<b>Nymark, M.; Sharma, A.K.; Sparstad, T.; Bones, A.M.; Winge, P.</b> (2016). A CRISPR/Cas9 system adapted for gene editing in marine algae. <i>NPG Scientific Reports 6(24951)</i>: 6 pp. <a href=\"http://dx.doi.org/10.1038/srep24951\" target=\"_blank\">http://dx.doi.org/10.1038/srep24951</a>","BEntID":247369,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 6(24951)</i>: 6 pp. <a href=\"http://dx.doi.org/10.1038/srep24951\" target=\"_blank\">http://dx.doi.org/10.1038/srep24951</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Nymark, M.; Sharma, A.K.; Sparstad, T.; Bones, A.M.; Winge, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Nymark, M. <i>et al.</i>","Englishabstract":"Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A CRISPR/Cas9 system adapted for gene editing in marine algae","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-15 01:32:49.487707","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2018-02-13","DateCreate":"2016-04-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000374656000001","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/srep24951"},"refs":null,"anarec":{"AnaID":255366,"PubliDate":2016,"Pagination":"6 pp","XtraPublOfAnaID":null,"ISBN":null,"Volume":"6","Issue":"24951","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). 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ISSN 2045-2322; e-ISSN 2045-2322","StandardTitleSer":"Scientific Reports (Nature Publishing Group)","ISSN":"2045-2322","AbbrevSer":"NPG Scientific Reports","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Nymark","Firstname":"Marianne","Initials":"M.","Affiliation":"Norwegian Univ Sci & Technol, Dept Biol, N-7491 Trondheim, Norway.","Discriminator":null,"CorporateFlag":0,"BEntID":247369,"AutID":299185,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Sharma","Firstname":"Amit Kumar","Initials":"A.K.","Affiliation":"Norwegian Univ Sci & Technol, Dept Biol, N-7491 Trondheim, Norway.","Discriminator":null,"CorporateFlag":0,"BEntID":247369,"AutID":299186,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Sparstad","Firstname":"Torfinn","Initials":"T.","Affiliation":"Norwegian Univ Sci & Technol, Dept Biol, N-7491 Trondheim, Norway.","Discriminator":null,"CorporateFlag":0,"BEntID":247369,"AutID":299187,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bones","Firstname":"Atle","Initials":"A.M.","Affiliation":"Norwegian Univ Sci & Technol, Dept Biol, N-7491 Trondheim, Norway.","Discriminator":null,"CorporateFlag":0,"BEntID":247369,"AutID":299188,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Winge","Firstname":"Per","Initials":"P.","Affiliation":"Norwegian Univ Sci & Technol, Dept Biol, N-7491 Trondheim, Norway.","Discriminator":null,"CorporateFlag":0,"BEntID":247369,"AutID":299189,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":289639,"LibID":36,"BRefID":255366,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"289639","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Nature Publishing Group","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":247369,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"http://dx.doi.org/10.1038/srep24951","externalID":"10.1038/srep24951","URLTypeCode":"DOI","URLID":45033,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2016-04-29 09:40:07.720000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Lyssens, Liesbeth, L.","updSesDate":{"date":"2018-02-13 19:34:46.407000","timezone_type":3,"timezone":"Europe/Brussels"}}}