{"refrec":{"BRefID":255806,"RR":"<b>Seternes, T.; Tonheim, T.C.; Myhr, A.I.; Dalmo, R.A.</b> (2016). A plant 35S CaMV promoter induces long-term expression of luciferase in Atlantic salmon. <i>NPG Scientific Reports 6(25096)</i>: 6 pp. <a href=\"http://dx.doi.org/10.1038/srep25096\" target=\"_blank\">http://dx.doi.org/10.1038/srep25096</a>","BEntID":247815,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 6(25096)</i>: 6 pp. <a href=\"http://dx.doi.org/10.1038/srep25096\" target=\"_blank\">http://dx.doi.org/10.1038/srep25096</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Seternes, T.; Tonheim, T.C.; Myhr, A.I.; Dalmo, R.A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Seternes, T. <i>et al.</i>","Englishabstract":"The long-term persistence and activity of a naked plasmid DNA (pGL3-35S) containing a luc gene (reporter gene) controlled by a plant 35S CaMV promoter was studied in Atlantic salmon (Salmo salar L.) after injection. Atlantic salmon (mean weight 70 grams) were injected intramuscularly with 100 mu g of plasmid DNA. Blood, different tissues and organs were sampled at different time points up to day 535 after injection. Southern blot analysis suggested the presence of extra-chromosomally open circular, linear and supercoiled topoforms of pGL3-35S at day 150 after injection. At day 536 open circular and supercoiled topoforms were detected. Luciferase activity was detected at the injection site up to 536 days post-injection of pGL3-35S, where it peaked at day 150 and decreased to approximately 17% of its maximum activity by day 536. Our study demonstrated that a plasmid containing the 35S promoter was able to induce expression of a reporter gene/protein in fish in vivo and that the plasmid DNA persisted for a prolonged time after intramuscular injection.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A plant 35S CaMV promoter induces long-term expression of luciferase in Atlantic salmon","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-15 01:32:49.956501","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2018-02-13","DateCreate":"2016-05-12","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000374986600001","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/srep25096"},"refs":null,"anarec":{"AnaID":255806,"PubliDate":2016,"Pagination":"6 pp","XtraPublOfAnaID":null,"ISBN":null,"Volume":"6","Issue":"25096","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). 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