{"refrec":{"BRefID":255814,"RR":"<b>Han, Y.; Liu, X.; Shi, B.; Xiao, R.; Gou, M.; Wang, H.; Li, Q.</b> (2016). Identification and characterisation of the immune response properties of <i>Lampetra japonica</i> BLNK. <i>NPG Scientific Reports 6(25308)</i>: 8 pp. <a href=\"http://dx.doi.org/10.1038/srep25308\" target=\"_blank\">http://dx.doi.org/10.1038/srep25308</a>","BEntID":247823,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 6(25308)</i>: 8 pp. <a href=\"http://dx.doi.org/10.1038/srep25308\" target=\"_blank\">http://dx.doi.org/10.1038/srep25308</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Han, Y.; Liu, X.; Shi, B.; Xiao, R.; Gou, M.; Wang, H.; Li, Q.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Han, Y. <i>et al.</i>","Englishabstract":"B cell linker protein (BLNK) is a central linker protein involved in B cell signal transduction in jawed vertebrates. In a previous study, we have reported the identification of a BLNK homolog named Lj-BLNK in lampreys. In this study, a 336 bp cDNA fragment encoding the Lj-BLNK Src homology 2 (SH2) domain was cloned into the vector pET-28a(+) and overexpressed in Escherichia coli BL21. The recombinant fragment of Lj-BLNK (rLj-BLNK) was purifiedby His-Bind affinity chromatography, and polyclonal antibodies against rLj-BLNK were raised in male New Zealand rabbits. Fluorescenceactivated cell sorting (FACS) analysis revealed that Lj-BLNK was expressed in approximately 48% of the lymphocyte-like cells of control lampreys, and a significant increase in Lj-BLNK expression was observed in lampreys stimulated with lipopolysaccharide (LPS). Western blotting analysis showed that variable lymphocyte receptor B (VLRB) and Lj-BLNKwere distributed in the same immune-relevant tissues, and the levels of both were upregulated in supraneural myeloid bodies and lymphocyte-like cells after LPS stimulation. Immunofluorescence demonstrated that Lj-BLNK was localized in VLRB+ lymphocyte-like cells. 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