{"refrec":{"BRefID":256709,"RR":"<b>Tuan, V.V.; Dantas-Lima, J.J.; Thuong, K.V.; Li, W.; Grauwet, K.; Bossier, P.; Nauwynck, H.</b> (2016). Differences in uptake and killing of pathogenic and non-pathogenic bacteria by haemocyte subpopulations of penaeid shrimp, <i>Litopenaeus vannamei</i>, (Boone). <i>J. Fish Dis. 39(2)</i>: 163-174. <a href=\"https://dx.doi.org/10.1111/jfd.12342\" target=\"_blank\">https://dx.doi.org/10.1111/jfd.12342</a>","BEntID":248718,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Fish Dis. 39(2)</i>: 163-174. <a href=\"https://dx.doi.org/10.1111/jfd.12342\" target=\"_blank\">https://dx.doi.org/10.1111/jfd.12342</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tuan, V.V.; Dantas-Lima, J.J.; Thuong, K.V.; Li, W.; Grauwet, K.; Bossier, P.; Nauwynck, H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tuan, V.V. <i>et al.</i>","Englishabstract":"Phagocytosis is an important function of both invertebrate and vertebrate blood cells. In this study, the phagocytic activity of haemocyte subpopulations of penaeid shrimp, <i>Litopenaeus vannamei</i>, (Boone), against pathogenic and non-pathogenic particles was investigated <i>in vitro</i>. The haemocytes of penaeid shrimp were firstly separated by centrifugation on a continuous density gradient of iodixanol into four fractions with five subpopulations (sub), of which sub 1 (hyalinocytes) and sub 4 (semi-granulocytes) have the main function in phagocytosis of both pathogenic and non-pathogenic bacteria as well as fluorescent polystyrene beads. It was found that these haemocyte subpopulations engulfed virulent <i>Vibrio campbellii</i> and <i>Vibrio harveyi</i> at a higher rate than non-virulent <i>Escherichia coli</i> and polystyrene beads. When these bacteria were mixed with shrimp haemocyte subpopulations and incubated for 180 min, the percentage of viable intracellular <i>V. campbellii</i> (25.5 ± 6.0%) recovered was significantly higher than the percentage recovered from <i>V. harveyi</i> (13.5 ± 1.1%). No viable intracellular <i>E. coli</i> was observed in this study. In contrast to <i>V. harveyi</i> and <i>E. coli</i>,<i> V. campbellii</i> containing endosomes did not acidify in time. Incubation of haemocyte subpopulations with the most virulent <i>V. campbellii</i> strain resulted in a significant drop in haemocyte viability (41.4 ± 6.3% in sub 1 and 30.2 ± 15.1% in sub 4) after 180 min post-inoculation in comparison with the less virulent <i>V. harveyi</i> (84.1 ± 5.6% in sub 1 and 83.4 ± 4.1% in sub 4) and non-virulent <i>E. coli</i> (92.7 ± 2.8% in sub 1 and 92.3 ± 5.6% in sub 4) and polystyrene beads (91.9 ± 1.6% in sub 1 and 84.4 ± 3.4% in sub 4). These findings may be a valuable tool for monitoring shrimp health and immunological studies.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Differences in uptake and killing of pathogenic and non-pathogenic bacteria by haemocyte subpopulations of penaeid shrimp, <i>Litopenaeus vannamei</i>, (Boone)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"bacteria; haemocyte subpopulations; Litopenaeus vannamei; phagocytosis","OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2018-08-28","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000370064400005","VABBcode":null,"OpenAcc":0,"DOI":"10.1111/jfd.12342"},"refs":null,"anarec":{"AnaID":256709,"PubliDate":2016,"Pagination":"163-174","XtraPublOfAnaID":null,"ISBN":null,"Volume":"39","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":44476,"SerRR":"Journal of Fish Diseases. Blackwell Science: Oxford; London; Edinburgh; Boston; Melbourne.  ISSN 0140-7775; e-ISSN 1365-2761","StandardTitleSer":"Journal of Fish Diseases","ISSN":"0140-7775","AbbrevSer":"J. 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