{"refrec":{"BRefID":256746,"RR":"<b>Dupont, A.; De Pauw-Gillet, M.-C.; Schnitzler, J.; Siebert, U.; Das, K.</b> (2016). Effects of methylmercury on harbour seal peripheral blood leucocytes in vitro studied by electron microscopy. <i>Arch. Environ. Contam. Toxicol. 70(1)</i>: 133-142. <a href=\"https://dx.doi.org/10.1007/s00244-015-0207-y\" target=\"_blank\">https://dx.doi.org/10.1007/s00244-015-0207-y</a>","BEntID":248755,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Arch. Environ. Contam. Toxicol. 70(1)</i>: 133-142. <a href=\"https://dx.doi.org/10.1007/s00244-015-0207-y\" target=\"_blank\">https://dx.doi.org/10.1007/s00244-015-0207-y</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Dupont, A.; De Pauw-Gillet, M.-C.; Schnitzler, J.; Siebert, U.; Das, K.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Dupont, A. <i>et al.</i>","Englishabstract":"Methylmercury (MeHg) is highly immunotoxic and can alter the health status of the harbour seal, <i>Phoca vitulina,</i> from the North Sea. To investigate the mechanism of MeHg-induced toxicity in harbour seal lymphocytes, Concanavalin A (ConA)-stimulated peripheral blood leucocytes were exposed in vitro to sublethal concentrations of MeHgCl (0.2, 1, and 2 µM) for 72 h and then analysed for their viability and ultrastructure. After 72 h of incubation, cells were counted with a propidium iodide staining technique, a metabolic MTS assay was performed, and cells exposed to 1 µM of MeHgCl were observed by transmission electron microscopy (TEM). Alive cell numbers decreased with increased MeHgCl concentrations. In presence of ConA and 1 µM of MeHgCl, TEM images revealed a higher frequency of apoptotic cells. Exposed cells displayed condensation of the chromatin at the nuclear membrane and mitochondrial damages. The results suggest that in vitro MeHgCl-induced apoptosis in harbour seal lymphocytes through a mitochondrial pathway.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Effects of methylmercury on harbour seal peripheral blood leucocytes in vitro studied by electron microscopy","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2018-08-28","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000368721800012","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s00244-015-0207-y"},"refs":null,"anarec":{"AnaID":256746,"PubliDate":2016,"Pagination":"133-142","XtraPublOfAnaID":null,"ISBN":null,"Volume":"70","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45321,"SerRR":"Archives of Environmental Contamination and Toxicology. 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