{"refrec":{"BRefID":256857,"RR":"<b>Delroisse, J.; Ortega-Martinez, O.; Dupont, S.; Mallefet, J.; Flammang, P.</b> (2015). De novo transcriptome of the European brittle star <i>Amphiura filiformis</i> pluteus larvae. <i>Marine Genomics 23</i>: 109-121. <a href=\"https://dx.doi.org/10.1016/j.margen.2015.05.014\" target=\"_blank\">https://dx.doi.org/10.1016/j.margen.2015.05.014</a>","BEntID":248866,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Marine Genomics 23</i>: 109-121. <a href=\"https://dx.doi.org/10.1016/j.margen.2015.05.014\" target=\"_blank\">https://dx.doi.org/10.1016/j.margen.2015.05.014</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Delroisse, J.; Ortega-Martinez, O.; Dupont, S.; Mallefet, J.; Flammang, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Delroisse, J. <i>et al.</i>","Englishabstract":"Background: In non-classical model species, Next Generation Sequencing increases the ability to analyze the expression of transcripts/genes. In this study, paired-end Illumina HiSeq sequencing technology has been employed to describe a larval transcriptome generated from 64 h post-fertilization pluteus larvae of the brittle star <i>Amphiura filiformis</i>. We focused our analysis on the detection of actors involved in the opsin based light perception, respectively the opsins and the phototransduction actors. Methods & results: In this research, about 47 million high quality reads were generated and 86,572 total unigenes were predicted after de novo assembly. Of all the larval unigenes, 18% show significant matches with reference online databases. 46% of annotated larval unigenes were significantly similar to transcripts from the purple sea urchin. COG, GO and KEGG analyses were performed on predicted unigenes. Regarding the opsin-based photoreception process, even if possible actors of ciliary and rhabdomeric phototransduction cascades were detected, no ciliary or rhabdomeric opsin was identified in these larvae. Additionally, partial non-visual RGR (retinal G protein coupled receptor) opsin mRNAs were identified,possibly indicating the presence of visual cycle reaction in early pluteus larvae. The eye morphogene Pax 6 was also identified in the pluteus transcriptome. Conclusions: Contrary to sea-urchin larvae, brittle star larvae appear to be characterized by an absence of visual-like opsins. These RNA-seq data also provide a useful resource for the echinoderm research community and researchers with an interest in larval biology.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"De novo transcriptome of the European brittle star <i>Amphiura filiformis</i> pluteus larvae","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Transcriptome; Echinodermata; Larval stage; Paired-end IlluminaSequencing; Photoreception; RGR opsin","OtherDescriptors":null,"Notes":null,"AnaPub":2015,"MonPub":null,"DateUpdate":"2018-08-31","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000362618000020","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.margen.2015.05.014"},"refs":null,"anarec":{"AnaID":256857,"PubliDate":2015,"Pagination":"109-121","XtraPublOfAnaID":null,"ISBN":null,"Volume":"23","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":126432,"SerRR":"Marine Genomics. 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