{"refrec":{"BRefID":256932,"RR":"<b>Orellana, G.; Van Meulebroek, L.; Van Vooren, S.; De Rijcke, M.; Vandegehuchte, M.; Janssen, C.R.; Vanhaecke, L.</b> (2015). Quantification and profiling of lipophilic marine toxins in microalgae by UHPLC coupled to high-resolution orbitrap mass spectrometry. <i>Anal. Bioanal. Chem. 407(21)</i>: 6345-6356. <a href=\"https://dx.doi.org/10.1007/s00216-015-8637-y\" target=\"_blank\">https://dx.doi.org/10.1007/s00216-015-8637-y</a>","BEntID":248941,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Anal. Bioanal. Chem. 407(21)</i>: 6345-6356. <a href=\"https://dx.doi.org/10.1007/s00216-015-8637-y\" target=\"_blank\">https://dx.doi.org/10.1007/s00216-015-8637-y</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Orellana, G.; Van Meulebroek, L.; Van Vooren, S.; De Rijcke, M.; Vandegehuchte, M.; Janssen, C.R.; Vanhaecke, L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Orellana, G. <i>et al.</i>","Englishabstract":"During the last decade, a significant increase in the occurrence of harmful algal blooms (HABs), linked to repetitive cases of shellfish contamination has become a public health concern and therefore, accurate methods to detect marine toxins in different matrices are required. In this study, we developed a method for profiling lipophilic marine microalgal toxins based on ultra-high-performance liquid chromatography coupled to high-resolution Orbitrap mass spectrometry (UHPLC-HR-Orbitrap MS). Extraction of selected toxins (okadaic acid (OA), dinophysistoxin-1 (DTX-1), pectenotoxin-2 (PTX-2), azaspiracid-1 (AZA-1), yessotoxin (YTX) and 13-desmethyl spirolide C (SPX-1)) was optimized using a Plackett-Burman design. Three key algal species, i.e., <i>Prorocentrum lima</i>, <i>Protoceratium reticulatum</i> and <i>Alexandrium ostenfeldii</i> were used to test the extraction efficiency of OA, YTXs and SPXs, respectively. <i>Prorocentrum micans</i>, fortified with certified reference solutions, was used for recovery studies. The quantitative and confirmatory performance of the method was evaluated according to CD 2002/657/EC. Limits of detection and quantification ranged between 0.006 and 0.050 ng mL<sup>-1</sup> and 0.018 to 0.227 ng mL<sup>-1</sup>, respectively. The intra-laboratory reproducibility ranged from 6.8 to 11.7 %, repeatability from 6.41 to 11.5 % and mean corrected recoveries from 81.9 to 119.6 %. In addition, algae cultures were retrospectively screened for analogues and metabolites through a homemade database. Using the ToxID software programme, 18 toxin derivates were detected in the extract of three toxin producing microalgae species. In conclusion, the generic extraction and full-scan HRMS approach offers an excellent quantitative performance and simultaneously allows to profile analogues and metabolites of marine toxins in microalgae.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Quantification and profiling of lipophilic marine toxins in microalgae by UHPLC coupled to high-resolution orbitrap mass spectrometry","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Ultra-high-performance liquid chromatography high-resolution Orbitrapmass spectrometry; Plackett-Burman; Validation; Harmful algae;Yessotoxins; Spirolide","OtherDescriptors":null,"Notes":null,"AnaPub":2015,"MonPub":null,"DateUpdate":"2018-03-08","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000358646400010","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s00216-015-8637-y"},"refs":null,"anarec":{"AnaID":256932,"PubliDate":2015,"Pagination":"6345-6356","XtraPublOfAnaID":null,"ISBN":null,"Volume":"407","Issue":"21","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":65810,"SerRR":"Analytical and Bioanalytical Chemistry. Springer: Heidelberg.  ISSN 1618-2642; e-ISSN 1618-2650","StandardTitleSer":"Analytical and Bioanalytical Chemistry","ISSN":"1618-2642","AbbrevSer":"Anal. Bioanal. 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