{"refrec":{"BRefID":257057,"RR":"<b>Zaiko, A.; Martinez, J.; Schmidt-Petersen, J.; Ribicic, D.; Samuiloviene, A.; Garcia-Vazquez, E.</b> (2015). Metabarcoding approach for the ballast water surveillance - An advantageous solution or an awkward challenge? <i>Mar. Pollut. Bull. 92(1-2)</i>: 25-34. <a href=\"https://dx.doi.org/10.1016/j.marpolbul.2015.01.008\" target=\"_blank\">https://dx.doi.org/10.1016/j.marpolbul.2015.01.008</a>","BEntID":249066,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":" <i>Mar. Pollut. Bull. 92(1-2)</i>: 25-34. <a href=\"https://dx.doi.org/10.1016/j.marpolbul.2015.01.008\" target=\"_blank\">https://dx.doi.org/10.1016/j.marpolbul.2015.01.008</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Zaiko, A.; Martinez, J.; Schmidt-Petersen, J.; Ribicic, D.; Samuiloviene, A.; Garcia-Vazquez, E.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Zaiko, A. <i>et al.</i>","Englishabstract":"Transfer of organisms with ships' ballast water is recognized as a major pathway of non-indigenous species introduction and addressed in a few recent legislative initiatives. Among other they imply scientific and technical research and monitoring to be conducted in a efficient and reliable way. The recent development of DNA barcoding and metabarcoding technologies opens new opportunities for biodiversity and biosecurity surveillance. In the current study, the performance of metabarcoding approach was assessed in comparison to the conventional (visual) observations, during the en route experimental ballast water survey. Opportunities and limitations of the molecular method were identified from taxonomical datasets rendered by two molecular markers of different degree of universality the universal cytochrome oxydase sub-unit I gene and a fragment of RuBisCO gene. 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