{"refrec":{"BRefID":257059,"RR":"<b>Huysman, M.J.J.; Tanaka, A.; Bowler, C.; Vyverman, W.; De Veylder, L.</b> (2015). Functional characterization of the diatom cyclin-dependent kinase A2 as a mitotic regulator reveals plant-like properties in a non-green lineage. <i>BMC Plant Biol. 15</i>. <a href=\"http://dx.doi.org/10.1186/s12870-015-0469-6\" target=\"_blank\">dx.doi.org/10.1186/s12870-015-0469-6</a>","BEntID":249068,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>BMC Plant Biol. 15</i>. <a href=\"https://dx.doi.org/10.1186/s12870-015-0469-6\" target=\"_blank\">https://dx.doi.org/10.1186/s12870-015-0469-6</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Huysman, M.J.J.; Tanaka, A.; Bowler, C.; Vyverman, W.; De Veylder, L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Huysman, M.J.J. <i>et al.</i>","Englishabstract":"BackgroundCyclin-dependent kinases (CDKs) are crucial regulators of cell cycle progression in eukaryotes. The diatom <i>CDKA2</i> was originally assigned to the classical A-type CDKs, but its cell cycle phase-specific transcription at the G2-to-M phase transition is typical for plant-specific B-type CDKs.ResultsHere, we report the functional characterization of CDKA2 from the diatom <i>Phaeodactylum tricornutum</i>. Through a yeast two-hybrid library screen, CDKA2 was found to interact with the G2/M-specific CDK scaffolding factor CKS1. Localization of CDKA2 was found to be nuclear in interphase cells, while in cells undergoing cytokinesis, the signal extended to the cell division plane. In addition, overexpression of <i>CDKA2</i> induced an overall reduction in the cell growth rate. Expression analysis of cell cycle marker genes in the overexpression lines indicates that this growth reduction is primarily due to a prolongation of the mitotic phase.ConclusionsOur study indicates a role for CDKA2 during cell division in diatoms. The functional characterization of a CDK with clear CDKB properties in a non-green organism questions whether the current definition of B-type CDKs being plant-specific might need revision.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Functional characterization of the diatom cyclin-dependent kinase A2 as a mitotic regulator reveals plant-like properties in a non-green lineage","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Cyclin-dependent kinase; CDKA2; Cell cycle; Cell division; Cytokinesis;Diatom; Mitosis; Phaeodactylum tricornutum","OtherDescriptors":null,"Notes":null,"AnaPub":2015,"MonPub":null,"DateUpdate":"2016-06-03","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000352468000001","VABBcode":null,"OpenAcc":1,"DOI":"10.1186/s12870-015-0469-6"},"refs":null,"anarec":{"AnaID":257059,"PubliDate":2015,"Pagination":null,"XtraPublOfAnaID":null,"ISBN":null,"Volume":"15","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208513,"SerRR":"BMC Plant Biology. 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ISSN 1471-2229; e-ISSN 1471-2229","StandardTitleSer":"BMC Plant Biology","ISSN":"1471-2229","AbbrevSer":"BMC Plant Biol.","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Huysman","Firstname":"Marie","Initials":"M.J.J.","Affiliation":"Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB)","Discriminator":null,"CorporateFlag":0,"BEntID":249068,"AutID":163060,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"PSB","InsFSN":"Flanders Institute for Biotechnology; VIB-Ugent Center for Plant Systems Biology","ORCID":null,"PersID":11965,"InsID":7968},{"AutName":"Tanaka","Firstname":null,"Initials":"A.","Affiliation":"Ecole Normale Super, CNRS, Inst Biol,Environm & Evolutionary Genom Sect, INSERM,Unite Mixte Rech 8186,U1024, F-75230 Paris 05, France.","Discriminator":null,"CorporateFlag":0,"BEntID":249068,"AutID":221922,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bowler","Firstname":null,"Initials":"C.","Affiliation":"Ecole Normale Super, CNRS, Inst Biol,Environm & Evolutionary Genom Sect, INSERM,Unite Mixte Rech 8186,U1024, F-75230 Paris 05, France.","Discriminator":null,"CorporateFlag":0,"BEntID":249068,"AutID":221923,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Vyverman","Firstname":"Wim","Initials":"W.","Affiliation":"Protistology and Aquatic Ecology, Department of Biology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":249068,"AutID":163073,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"PAE","InsFSN":"Universiteit Gent; 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