{"refrec":{"BRefID":257197,"RR":"<b>Stalmans, S.; Willems, M.; Adriaens, E.; Remon, J.-P.; D'Hondt, M.; De Spiegeleer, B.</b> (2014). Flatworm models in pharmacological research: the importance of compound stability testing. <i>Regul. Toxicol. Pharmacol. 70(1)</i>: 149-154. <a href=\"https://dx.doi.org/10.1016/j.yrtph.2014.06.026\" target=\"_blank\">https://dx.doi.org/10.1016/j.yrtph.2014.06.026</a>","BEntID":249206,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Regul. Toxicol. Pharmacol. 70(1)</i>: 149-154. <a href=\"https://dx.doi.org/10.1016/j.yrtph.2014.06.026\" target=\"_blank\">https://dx.doi.org/10.1016/j.yrtph.2014.06.026</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Stalmans, S.; Willems, M.; Adriaens, E.; Remon, J.-P.; D'Hondt, M.; De Spiegeleer, B.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Stalmans, S. <i>et al.</i>","Englishabstract":"Flatworms possess adult pluripotent stem cells, which make them extraordinary experimental model organisms to assess <i>in vivo</i> the undesirable effects of substances on stem cells. Currently, quality practices, implying evaluation of the stability of the test compound under the proposed experimental conditions, are uncommon in this research field. Nevertheless, performing a stability study during the rational design of <i>in vivo</i> assay protocols will result in more reliable assay results. To illustrate the influence of the stability of the test substance on the final experimental outcome, we performed a short-term International Conference on Harmonization (ICH)-based stability study of cyclophosphamide in the culture medium, to which a marine flatworm model <i>Macrostomum lignano</i> is exposed. Using a validated U(H)PLC method, it was demonstrated that the cyclophosphamide concentration in the culture medium at 20 °C is lowered to 80% of the initial concentration after 21 days. The multiwell plates, flatworms and diatoms, as well as light exposure, did not influence significantly the cyclophosphamide concentration in the medium. The results of the stability study have practical implications on the experimental set-up of the carcinogenicity assay like the frequency of medium renewal. This case study demonstrates the benefits of applying appropriate quality guidelines already during fundamental research increasing the credibility of the results.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Flatworm models in pharmacological research: the importance of compound stability testing","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Assay design; Flatworms; Quality practices; Stability testing; Stemcells; Toxico-pharmacological test; U(H)PLC","OtherDescriptors":null,"Notes":null,"AnaPub":2014,"MonPub":null,"DateUpdate":"2019-08-29","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000342482200016","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.yrtph.2014.06.026"},"refs":null,"anarec":{"AnaID":257197,"PubliDate":2014,"Pagination":"149-154","XtraPublOfAnaID":null,"ISBN":null,"Volume":"70","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":227256,"SerRR":"Regulatory Toxicology and Pharmacology. Elsevier: New York, N.Y..  ISSN 0273-2300; e-ISSN 1096-0295","StandardTitleSer":"Regulatory Toxicology and Pharmacology","ISSN":"0273-2300","AbbrevSer":"Regul. Toxicol. Pharmacol.","StandardTitleMon":null,"StartPage":149,"Pages":6,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Stalmans","Firstname":"Sofie","Initials":"S.","Affiliation":"Drug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":249206,"AutID":222782,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"DruQuaR","InsFSN":"Universiteit Gent; Faculteit Farmaceutische Wetenschappen; Vakgroep Farmaceutische Analyse; Drug Quality and Registration","ORCID":null,"PersID":34899,"InsID":13976},{"AutName":"Willems","Firstname":"Maxime","Initials":"M.","Affiliation":"Laboratory of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":249206,"AutID":222783,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Farmaceutische Wetenschappen; Laboratorium voor Farmaceutische Technologie","ORCID":null,"PersID":30141,"InsID":12132},{"AutName":"Adriaens","Firstname":"Els","Initials":"E.","Affiliation":"Laboratory of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":249206,"AutID":222784,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Farmaceutische Wetenschappen; Laboratorium voor Farmaceutische Technologie","ORCID":null,"PersID":null,"InsID":12132},{"AutName":"Remon","Firstname":"Jean-Paul","Initials":"J.-P.","Affiliation":"Laboratory of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":249206,"AutID":222785,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Farmaceutische Wetenschappen; Laboratorium voor Farmaceutische Technologie","ORCID":null,"PersID":597,"InsID":12132},{"AutName":"D'Hondt","Firstname":"Matthias","Initials":"M.","Affiliation":"Drug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":249206,"AutID":222786,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"DruQuaR","InsFSN":"Universiteit Gent; Faculteit Farmaceutische Wetenschappen; Vakgroep Farmaceutische Analyse; Drug Quality and Registration","ORCID":null,"PersID":34900,"InsID":13976},{"AutName":"De Spiegeleer","Firstname":"Bart","Initials":"B.","Affiliation":"Drug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":249206,"AutID":222787,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"DruQuaR","InsFSN":"Universiteit Gent; Faculteit Farmaceutische Wetenschappen; Vakgroep Farmaceutische Analyse; Drug Quality and Registration","ORCID":"0000-0001-6794-3108","PersID":34852,"InsID":13976}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":335987,"LibID":36,"BRefID":257197,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"335987","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":"New York, N.Y."}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Macrostomum lignano"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":249206,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":249206,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.yrtph.2014.06.026","externalID":"10.1016/j.yrtph.2014.06.026","URLTypeCode":"DOI","URLID":55894,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Macrostomum lignano","AphiaID":484258,"TaxtID":37154}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Macrostomum lignano","DutchTerm":null,"AphiaID":484258,"TaxtID":37154}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2016-05-29 23:09:30.633000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Verheyde, Fons, F.","updSesDate":{"date":"2019-08-29 07:51:26.000000","timezone_type":3,"timezone":"Europe/Brussels"}}}