{"refrec":{"BRefID":257209,"RR":"<b>Logares, R.; Sunagawa, S.; Salazar, G.; Cornejo-Castillo, F.; Ferrera, I.; Sarmento, H.; Hingamp, P.; Ogata, H.; de Vargas, C.; Lima-Mendez, G.; Raes, J.; Poulain, J.; Jaillon, O.; Wincker, P.; Kandels-Lewis, S.; Karsenti, E.; Bork, P.; Acinas, S.</b> (2014). Metagenomic 16S rDNA Illumina tags are a powerful alternative to amplicon sequencing to explore diversity and structure of microbial communities. <i>Environ. Microbiol. 16(9)</i>: 2659-2671. <a href=\"https://dx.doi.org/10.1111/1462-2920.12250\" target=\"_blank\">https://dx.doi.org/10.1111/1462-2920.12250</a>","BEntID":249218,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Environ. Microbiol. 16(9)</i>: 2659-2671. <a href=\"https://dx.doi.org/10.1111/1462-2920.12250\" target=\"_blank\">https://dx.doi.org/10.1111/1462-2920.12250</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Logares, R.; Sunagawa, S.; Salazar, G.; Cornejo-Castillo, F.; Ferrera, I.; Sarmento, H.; Hingamp, P.; Ogata, H.; de Vargas, C.; Lima-Mendez, G.; Raes, J.; Poulain, J.; Jaillon, O.; Wincker, P.; Kandels-Lewis, S.; Karsenti, E.; Bork, P.; Acinas, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Logares, R. <i>et al.</i>","Englishabstract":"Sequencing of 16S rDNA polymerase chain reaction (PCR) amplicons is the most common approach for investigating environmental prokaryotic diversity, despite the known biases introduced during PCR. Here we show that 16S rDNA fragments derived from Illumina-sequenced environmental metagenomes (<i><sub>mi</sub>tag</i>s) are a powerful alternative to 16S rDNA amplicons for investigating the taxonomic diversity and structure of prokaryotic communities. As part of the <em>Tara</em> Oceans global expedition, marine plankton was sampled in three locations, resulting in 29 subsamples for which metagenomes were produced by shotgun Illumina sequencing (ca. 700?Gb). For comparative analyses, a subset of samples was also selected for Roche-454 sequencing using both shotgun (<i><sub>m454</sub>tag</i>s; 13 metagenomes, ca. 2.4?Gb) and 16S rDNA amplicon (<i><sub>454</sub>tag</i>s; ca. 0.075?Gb) approaches. Our results indicate that by overcoming PCR biases related to amplification and primer mismatch, <i><sub>mi</sub>tag</i>s may provide more realistic estimates of community richness and evenness than amplicon <i><sub>454</sub>tag</i>s. In addition, <i><sub>mi</sub>tag</i>s can capture expected beta diversity patterns. Using <i><sub>mi</sub>tag</i>s is now economically feasible given the dramatic reduction in high-throughput sequencing costs, having the advantage of retrieving simultaneously both taxonomic (Bacteria, Archaea and Eukarya) and functional information from the same microbial community.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Metagenomic 16S rDNA Illumina tags are a powerful alternative to amplicon sequencing to explore diversity and structure of microbial communities","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-19 01:31:42.483520","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2014,"MonPub":null,"DateUpdate":"2020-06-25","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000341579700004","VABBcode":null,"OpenAcc":0,"DOI":"10.1111/1462-2920.12250"},"refs":null,"anarec":{"AnaID":257209,"PubliDate":2014,"Pagination":"2659-2671","XtraPublOfAnaID":null,"ISBN":null,"Volume":"16","Issue":"9","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":59926,"SerRR":"Environmental Microbiology. Blackwell Scientific Publishers: Oxford.  ISSN 1462-2912; e-ISSN 1462-2920","StandardTitleSer":"Environmental Microbiology","ISSN":"1462-2912","AbbrevSer":"Environ. Microbiol.","StandardTitleMon":null,"StartPage":2659,"Pages":13,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Logares","Firstname":null,"Initials":"R.","Affiliation":"CSIC, Dept Marine Biol & Oceanog, Inst Marine Sci ICM, ES-08003 Barcelona, Spain.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222874,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Sunagawa","Firstname":null,"Initials":"S.","Affiliation":"European Mol Biol Lab, D-69117 Heidelberg, Germany.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222875,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Salazar","Firstname":null,"Initials":"G.","Affiliation":"CSIC, Dept Marine Biol & Oceanog, Inst Marine Sci ICM, ES-08003 Barcelona, Spain.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222876,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Cornejo-Castillo","Firstname":null,"Initials":"F.","Affiliation":"CSIC, Dept Marine Biol & Oceanog, Inst Marine Sci ICM, ES-08003 Barcelona, Spain.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222877,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Ferrera","Firstname":null,"Initials":"I.","Affiliation":"CSIC, Dept Marine Biol & Oceanog, Inst Marine Sci ICM, ES-08003 Barcelona, Spain.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222878,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Sarmento","Firstname":null,"Initials":"H.","Affiliation":"CSIC, Dept Marine Biol & Oceanog, Inst Marine Sci ICM, ES-08003 Barcelona, Spain.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222879,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":"0000-0001-5220-7992","PersID":27631,"InsID":null},{"AutName":"Hingamp","Firstname":null,"Initials":"P.","Affiliation":"Aix Marseille Univ, Inst Microbiol Mediterranee, Ctr Natl Rech Sci, F-13288 Marseille, France.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222881,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Ogata","Firstname":null,"Initials":"H.","Affiliation":"Aix Marseille Univ, Inst Microbiol Mediterranee, Ctr Natl Rech Sci, F-13288 Marseille, France.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222882,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"de Vargas","Firstname":null,"Initials":"C.","Affiliation":"Univ Paris 06, Stn Biol Roscoff, Ctr Natl Rech Sci, Unite Mixte Rech 7144, FR-29682 Roscoff, France.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222884,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Lima-Mendez","Firstname":"Gipsi","Initials":"G.","Affiliation":"Research Group of Bioinformatics and (Eco-)Systems biology, Department of Structural Biology, Vlaams Institut voor Biotechnologie","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222885,"OrderNr":10,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":"0000-0002-5309-1845","PersID":31532,"InsID":null},{"AutName":"Raes","Firstname":"Jeroen","Initials":"J.","Affiliation":"Research Group of Bioinformatics and (Eco-)Systems biology, Department of Structural Biology, Vlaams Institut voor Biotechnologie","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222887,"OrderNr":11,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":"0000-0002-1337-041X","PersID":31527,"InsID":null},{"AutName":"Poulain","Firstname":null,"Initials":"J.","Affiliation":"Commissariat Energie Atom, Inst Genom, Genoscope, F-91057 Evry, France.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222889,"OrderNr":12,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Jaillon","Firstname":null,"Initials":"O.","Affiliation":"Commissariat Energie Atom, Inst Genom, Genoscope, F-91057 Evry, France.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222890,"OrderNr":13,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Wincker","Firstname":null,"Initials":"P.","Affiliation":"Commissariat Energie Atom, Inst Genom, Genoscope, F-91057 Evry, France.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222893,"OrderNr":14,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Kandels-Lewis","Firstname":null,"Initials":"S.","Affiliation":"European Mol Biol Lab, D-69117 Heidelberg, Germany.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222896,"OrderNr":15,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Karsenti","Firstname":null,"Initials":"E.","Affiliation":"European Mol Biol Lab, D-69117 Heidelberg, Germany.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222897,"OrderNr":16,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bork","Firstname":null,"Initials":"P.","Affiliation":"European Mol Biol Lab, D-69117 Heidelberg, Germany.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222898,"OrderNr":17,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Acinas","Firstname":null,"Initials":"S.","Affiliation":"CSIC, Dept Marine Biol & Oceanog, Inst Marine Sci ICM, ES-08003 Barcelona, Spain.","Discriminator":null,"CorporateFlag":0,"BEntID":249218,"AutID":222899,"OrderNr":18,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":346570,"LibID":36,"BRefID":257209,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"346570","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Blackwell Scientific Publishers","City":"Oxford"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":249218,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":249218,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1111/1462-2920.12250","externalID":"10.1111/1462-2920.12250","URLTypeCode":"DOI","URLID":46555,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2016-05-29 23:09:30.633000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2020-06-25 08:07:04.923000","timezone_type":3,"timezone":"Europe/Brussels"}}}