{"refrec":{"BRefID":257296,"RR":"<b>Geraylou, Z.; Vanhove, M.P.M.; Souffreau, C.; Rurangwa, E.; Buyse, J.; Ollevier, F.</b> (2014). <i>In vitro</i> selection and characterization of putative probiotics isolated from the gut of <i>Acipenser baerii</i> (Brandt, 1869). <i>Aquac. Res. 45(2)</i>: 341-352. <a href=\"https://dx.doi.org/10.1111/j.1365-2109.2012.03232.x\" target=\"_blank\">https://dx.doi.org/10.1111/j.1365-2109.2012.03232.x</a>","BEntID":249305,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquac. Res. 45(2)</i>: 341-352. <a href=\"https://dx.doi.org/10.1111/j.1365-2109.2012.03232.x\" target=\"_blank\">https://dx.doi.org/10.1111/j.1365-2109.2012.03232.x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":1,"BrackishFlag":1,"TerrestrialFlag":0,"Authorstring":"Geraylou, Z.; Vanhove, M.P.M.; Souffreau, C.; Rurangwa, E.; Buyse, J.; Ollevier, F.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Geraylou, Z. <i>et al.</i>","Englishabstract":"To select and characterize potential probiotic bacteria from the gut microbiota of Siberian sturgeon (<i>Acipenser baerii</i>), 129 strains isolated from the hindgut were screened for antagonistic activity against five fish pathogens. Ten isolates showed antagonism towards three or more pathogens. Nine of these isolates were Gram-positive, belonging to <i>Lactococcus</i> (seven) and <i>Bacillus</i> (two), and a single strain belonging to the Gram-negative <i>Citrobacter</i>. These inhibitory isolates were identified using genetic, phentotypic and biochemical traits, and further characterized by <i>in vitro</i> tests assessing the adhesion and growth in mucus and resistance to gastric and intestinal fluids. The candidate probiotics were determined to be non-pathogenic through an <i>in vivo</i> study. Based on these assays<i>, Lactococcus lactis</i> ssp<i>. lactis </i> STG45 and STG81 showed the broadest inhibitory potential, a high viability in simulated gastrointestinal juice and the highest adhesion capacity to mucus. They were therefore selected as the most promising candidate probiotics. 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