{"refrec":{"BRefID":257448,"RR":"<b>Bellassoued, K.; Hamza, A.; Van Pelt, J.; Elfeki, A.</b> (2012). Evaluation of cytotoxic compounds in different organs of the sea bream <i>Sarpa salpa</i> as related to phytoplankton consumption: an in vitro study in human liver cell lines HepG2 and WRL68. <i>In Vitro Cell. Dev. Biol. A 48(8)</i>: 528-534. <a href=\"http://dx.doi.org/10.1007/s11626-012-9533-9\" target=\"_blank\">dx.doi.org/10.1007/s11626-012-9533-9</a>","BEntID":249457,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>In Vitro Cell. Dev. Biol. A 48(8)</i>: 528-534. <a href=\"https://dx.doi.org/10.1007/s11626-012-9533-9\" target=\"_blank\">https://dx.doi.org/10.1007/s11626-012-9533-9</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Bellassoued, K.; Hamza, A.; Van Pelt, J.; Elfeki, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Bellassoued, K. <i>et al.</i>","Englishabstract":"The present study was aimed to assess the cytotoxic effects of not-yet identified compounds present in organ extracts of Sarpa salpa, collected in autumn, the period with a peak in health problems. In addition, we studied the cytotoxicity of extracts of epiphytes found in the stomach content of S. salpa collected in summer and of epiphytes collected from the sea in the Sfax area at the end of spring. We tested these fractions in two human hepatic cell lines: HepG2 and WRL68. We observed a significant loss of viable cells when HepG2 cells were exposed for 72 h to acetone extracts of livers of S. salpa at a concentration of 2.5 mg/ml protein. Proteins extracted from brain or muscle did not significantly induce cell death at the studied concentrations (a parts per thousand currency sign10 mg/ml). There was a significant loss of viable cells when treated with liver extract of S. salpa dissolved in DMSO. Extracts of epiphytes collected in late spring showed a cytotoxic effect in a concentration-dependent manner. Moreover, we observed a significantly decreased cell viability of HepG2 at a dilution (1/40) of epiphyte extracts from stomach contents of two fish we had collected. The cytotoxic effect of the observed epiphyte extracts confirms the transfer of toxins originating from toxic dinoflagellates which live in epiphyte on the Posidonia oceanica leaves to fish organs by grazing. Hence, the liver of this fish can cause a threat to human health and consumption should for this reason be dissuaded.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Evaluation of cytotoxic compounds in different organs of the sea bream <i>Sarpa salpa</i> as related to phytoplankton consumption: an in vitro study in human liver cell lines HepG2 and WRL68","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Sarpa salpa; Organ extract; Epiphyte extract; WRL68; HepG2","OtherDescriptors":null,"Notes":null,"AnaPub":2012,"MonPub":null,"DateUpdate":"2016-06-10","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000309346000009","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s11626-012-9533-9"},"refs":null,"anarec":{"AnaID":257448,"PubliDate":2012,"Pagination":"528-534","XtraPublOfAnaID":null,"ISBN":null,"Volume":"48","Issue":"8","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":258581,"SerRR":"In Vitro Cellular & Developmental Biology - Animal. Springer: Berlin.  ISSN 1071-2690; e-ISSN 1543-706X","StandardTitleSer":"In Vitro Cellular & Developmental Biology - Animal","ISSN":"1071-2690","AbbrevSer":"In Vitro Cell. Dev. Biol. A","StandardTitleMon":null,"StartPage":528,"Pages":7,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Bellassoued","Firstname":null,"Initials":"K.","Affiliation":"Univ Sfax, Fac Sci, Lab Anim Ecophysiol, Sfax 3018, Tunisia.","Discriminator":null,"CorporateFlag":0,"BEntID":249457,"AutID":225053,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Hamza","Firstname":null,"Initials":"A.","Affiliation":"INSTM Sfax, Marine Biotechnol & Biodivers Lab, Sfax, Tunisia.","Discriminator":null,"CorporateFlag":0,"BEntID":249457,"AutID":225056,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Van Pelt","Firstname":null,"Initials":"J.","Affiliation":"Liver Research Facility/Labo Hepatology, University Hospital Gasthuisberg","Discriminator":null,"CorporateFlag":0,"BEntID":249457,"AutID":225057,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Elfeki","Firstname":null,"Initials":"A.","Affiliation":"Sci Fac Sfax, Dept Life Sci, Anim Ecophysiol Lab, Sfax 3000, Tunisia.","Discriminator":null,"CorporateFlag":0,"BEntID":249457,"AutID":225058,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":291676,"LibID":36,"BRefID":257448,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"291676","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open Marien Archief","URL":null,"ClassifID":235,"Classification":"Open Marine Archive","ReqLink":null,"ClassifTypID":5,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":0,"InternalReq":0,"LoggedInReq":0,"Disclaimer":"Disclaimer_OMA","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Springer","City":"Berlin"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Sarpa salpa"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":249457,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":249457,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1007/s11626-012-9533-9","externalID":"10.1007/s11626-012-9533-9","URLTypeCode":"DOI","URLID":46747,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Sarpa salpa","AphiaID":127064,"TaxtID":39632}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Sarpa salpa","DutchTerm":null,"AphiaID":127064,"TaxtID":39632}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2016-05-29 23:20:56.993000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2016-06-10 13:15:48.043000","timezone_type":3,"timezone":"Europe/Brussels"}}}