{"refrec":{"BRefID":257464,"RR":"<b>Defoirdt, T.; Benneche, T.; Brackman, G.; Coenye, T.; Sorgeloos, P.; Scheie, A.</b> (2012). A quorum sensing-disrupting brominated thiophenone with a promising therapeutic potential to treat luminescent vibriosis. <i>PLoS One 7(7)</i>: e41788. <a href=\"http://dx.doi.org/10.1371/journal.pone.0041788\" target=\"_blank\">dx.doi.org/10.1371/journal.pone.0041788</a>","BEntID":249473,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>PLoS One 7(7)</i>: e41788. <a href=\"https://dx.doi.org/10.1371/journal.pone.0041788\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0041788</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Defoirdt, T.; Benneche, T.; Brackman, G.; Coenye, T.; Sorgeloos, P.; Scheie, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Defoirdt, T. <i>et al.</i>","Englishabstract":"<i>Vibrio harveyi</i> is amongst the most important bacterial pathogens in aquaculture. Novel methods to control this pathogen are needed since many strains have acquired resistance to antibiotics. We previously showed that quorum sensing-disrupting furanones are able to protect brine shrimp larvae against vibriosis. However, a major problem of these compounds is that they are toxic toward higher organisms and therefore, they are not safe to be used in aquaculture. The synthesis of brominated thiophenones, sulphur analogues of the quorum sensing-disrupting furanones, has recently been reported. In the present study, we report that these compounds block quorum sensing in <i>V. harveyi</i> at concentrations in the low micromolar range. Bioluminescence experiments with <i>V. harveyi</i> quorum sensing mutants and a fluorescence anisotropy assay indicated that the compounds disrupt quorum sensing in this bacterium by decreasing the ability of the quorum sensing master regulator LuxR to bind to its target promoter DNA. <i>In vivo</i> challenge tests with gnotobiotic brine shrimp larvae showed that thiophenone compound TF310, (<i>Z</i>)-4-((5-(bromomethylene)-2-oxo-2,5-dihydrothiophen-3-yl)methoxy)-4-oxobutanoic acid, completely protected the larvae from <i>V. harveyi</i> BB120 when dosed to the culture water at 2.5 µM or more, whereas severe toxicity was only observed at 250 µM. This makes TF310 showing the highest therapeutic index of all quorum sensing-disrupting compounds tested thus far in our brine shrimp model system.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A quorum sensing-disrupting brominated thiophenone with a promising therapeutic potential to treat luminescent vibriosis","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:01.897972","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2012,"MonPub":null,"DateUpdate":"2016-06-15","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000306806600118","VABBcode":null,"OpenAcc":1,"DOI":"10.1371/journal.pone.0041788"},"refs":null,"anarec":{"AnaID":257464,"PubliDate":2012,"Pagination":"e41788","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":"7","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":123954,"SerRR":"PLoS One. 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