{"refrec":{"BRefID":257475,"RR":"<b>Vaulot, D.; Lepere, C.; Toulza, E.; De la Iglesia, R.; Poulain, J.; Gaboyer, F.; Moreau, H.; Vandepoele, K.; Ulloa, O.; Gavory, F.; Piganeau, G.</b> (2012). Metagenomes of the picoalga <i>Bathycoccus</i> from the Chile coastal upwelling. <i>PLoS One 7(6)</i>: e39648. <a href=\"http://dx.doi.org/10.1371/journal.pone.0039648\" target=\"_blank\">dx.doi.org/10.1371/journal.pone.0039648</a>","BEntID":249484,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>PLoS One 7(6)</i>: e39648. <a href=\"https://dx.doi.org/10.1371/journal.pone.0039648\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0039648</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Vaulot, D.; Lepere, C.; Toulza, E.; De la Iglesia, R.; Poulain, J.; Gaboyer, F.; Moreau, H.; Vandepoele, K.; Ulloa, O.; Gavory, F.; Piganeau, G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Vaulot, D. <i>et al.</i>","Englishabstract":"Among small photosynthetic eukaryotes that play a key role in oceanic food webs, picoplanktonic Mamiellophyceae such as <i>Bathycoccus</i>, <i>Micromonas</i>, and <i>Ostreococcus</i> are particularly important in coastal regions. By using a combination of cell sorting by flow cytometry, whole genome amplification (WGA), and 454 pyrosequencing, we obtained metagenomic data for two natural picophytoplankton populations from the coastal upwelling waters off central Chile. About 60% of the reads of each sample could be mapped to the genome of <i>Bathycoccus</i> strain from the Mediterranean Sea (RCC1105), representing a total of 9 Mbp (sample T142) and 13 Mbp (sample T149) of non-redundant <i>Bathycoccus</i> genome sequences. WGA did not amplify all regions uniformly, resulting in unequal coverage along a given chromosome and between chromosomes. The identity at the DNA level between the metagenomes and the cultured genome was very high (96.3% identical bases for the three larger chromosomes over a 360 kbp alignment). At least two to three different genotypes seemed to be present in each natural sample based on read mapping to <i>Bathycoccus</i> RCC1105 genome.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Metagenomes of the picoalga <i>Bathycoccus</i> from the Chile coastal upwelling","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2012,"MonPub":null,"DateUpdate":"2016-06-07","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000305730900092","VABBcode":null,"OpenAcc":1,"DOI":"10.1371/journal.pone.0039648"},"refs":null,"anarec":{"AnaID":257475,"PubliDate":2012,"Pagination":"e39648","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":"6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":123954,"SerRR":"PLoS One. 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