{"refrec":{"BRefID":257516,"RR":"<b>Verdoodt, F.; Willems, M.; Mouton, S.; De Mulder, K.; Bert, W.; Houthoofd, W.; Smith, J.; Ladurner, P.</b> (2012). Stem cells propagate their DNA by random segregation in the flatworm <i>Macrostomum lignano</i>. <i>PLoS One 7(1)</i>: e30227. <a href=\"http://dx.doi.org/10.1371/journal.pone.0030227\" target=\"_blank\">dx.doi.org/10.1371/journal.pone.0030227</a>","BEntID":249525,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>PLoS One 7(1)</i>: e30227. <a href=\"https://dx.doi.org/10.1371/journal.pone.0030227\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0030227</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Verdoodt, F.; Willems, M.; Mouton, S.; De Mulder, K.; Bert, W.; Houthoofd, W.; Smith, J.; Ladurner, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Verdoodt, F. <i>et al.</i>","Englishabstract":"Adult stem cells are proposed to have acquired special features to prevent an accumulation of DNA-replication errors. Two such mechanisms, frequently suggested to serve this goal are cellular quiescence, and non-random segregation of DNA strands during stem cell division, a theory designated as the immortal strand hypothesis. To date, it has been difficult to test the <i>in vivo</i> relevance of both mechanisms in stem cell systems. It has been shown that in the flatworm <i>Macrostomum lignano</i> pluripotent stem cells (neoblasts) are present in adult animals. We sought to address by which means <i>M. lignano</i> neoblasts protect themselves against the accumulation of genomic errors, by studying the exact mode of DNA-segregation during their division.In this study, we demonstrated four lines of <i>in vivo</i> evidence in favor of cellular quiescence. Firstly, performing BrdU pulse-chase experiments, we localized ‘Label-Retaining Cells’ (LRCs). Secondly, EDU pulse-chase combined with Vasa labeling demonstrated the presence of neoblasts among the LRCs, while the majority of LRCs were differentiated cells.We showed that stem cells lose their label at a slow rate, indicating cellular quiescence. Thirdly, CldU/IdU- double labeling studies confirmed that label-retaining stem cells showed low proliferative activity. Finally, the use of the actin inhibitor, cytochalasin D, unequivocally demonstrated random segregation of DNA-strands in LRCs.Altogether, our data unambiguously demonstrated that the majority of neoblasts in <i>M. lignano</i> distribute their DNA randomly during cell division, and that label-retention is a direct result of cellular quiescence, rather than a sign of co-segregation of labeled strands.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Stem cells propagate their DNA by random segregation in the flatworm <i>Macrostomum lignano</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2012,"MonPub":null,"DateUpdate":"2016-06-07","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000301457200045","VABBcode":null,"OpenAcc":1,"DOI":"10.1371/journal.pone.0030227"},"refs":null,"anarec":{"AnaID":257516,"PubliDate":2012,"Pagination":"e30227","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":123954,"SerRR":"PLoS One. 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