{"refrec":{"BRefID":257784,"RR":"<b>Robbins, H.; Van Stappen, G.; Sorgeloos, P.; Sung, Y.; MacRae, T.; Bossier, P.</b> (2010). Diapause termination and development of encysted <i>Artemia</i> embryos: roles for nitric oxide and hydrogen peroxide. <i>J. Exp. Biol. 213(9)</i>: 1464-1470. <a href=\"https://dx.doi.org/10.1242/jeb.041772\" target=\"_blank\">https://dx.doi.org/10.1242/jeb.041772</a>","BEntID":249794,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Exp. Biol. 213(9)</i>: 1464-1470. <a href=\"https://dx.doi.org/10.1242/jeb.041772\" target=\"_blank\">https://dx.doi.org/10.1242/jeb.041772</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Robbins, H.; Van Stappen, G.; Sorgeloos, P.; Sung, Y.; MacRae, T.; Bossier, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Robbins, H. <i>et al.</i>","Englishabstract":"Encysted embryos (cysts) of the brine shrimp <i>Artemia</i> undergo diapause, a state of profound dormancy and enhanced stress tolerance. Upon exposure to the appropriate physical stimulus diapause terminates and embryos resume development. The regulation of diapause termination and post-diapause development is poorly understood at the molecular level, prompting this study on the capacity of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and nitric oxide (NO) to control these processes. Exposure to H<sub>2</sub>O<sub>2</sub> and NO, the latter generated by the use of three NO generators, promoted cyst development, emergence and hatching, effects nullified by catalase and the NO scavenger 2-phenyl-4,4,5,5,-tetramethylimidazoline-1-oxyl 3-oxide (PTIO). The maximal effect of NO and H<sub>2</sub>O<sub>2</sub> on cyst development was achieved by 4 h of exposure to either chemical. NO was effective at a lower concentration than H<sub>2</sub>O<sub>2</sub> but more cysts developed in response to H<sub>2</sub>O<sub>2</sub>. Promotion of development varied with incubation conditions, indicating for the first time a population of <i>Artemia</i> cysts potentially arrested in post-diapause and whose development was activated by either H<sub>2</sub>O<sub>2</sub> or NO. A second cyst sub-population, refractory to hatching after prolonged incubation, was considered to be in diapause, a condition broken by H<sub>2</sub>O<sub>2</sub> but not NO. These observations provide clues to the molecular mechanisms of diapause termination and development in <i>Artemia</i>, while enhancing the organism's value in aquaculture by affording a greater understanding of its growth and physiology.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Diapause termination and development of encysted <i>Artemia</i> embryos: roles for nitric oxide and hydrogen peroxide","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-25 01:32:16.343934","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"diapause; nitric oxide; hydrogen peroxide; Artemia","OtherDescriptors":null,"Notes":null,"AnaPub":2010,"MonPub":null,"DateUpdate":"2019-12-23","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000276787200017","VABBcode":null,"OpenAcc":0,"DOI":"10.1242/jeb.041772"},"refs":null,"anarec":{"AnaID":257784,"PubliDate":2010,"Pagination":"1464-1470","XtraPublOfAnaID":null,"ISBN":null,"Volume":"213","Issue":"9","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43227,"SerRR":"The Journal of Experimental Biology. Cambridge University Press: London.  ISSN 0022-0949; e-ISSN 1477-9145","StandardTitleSer":"The Journal of Experimental Biology","ISSN":"0022-0949","AbbrevSer":"J. Exp. Biol.","StandardTitleMon":null,"StartPage":1464,"Pages":7,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Robbins","Firstname":null,"Initials":"H.","Affiliation":"Dalhousie Univ, Dept Biol, Halifax, NS B3H 4J1, Canada.","Discriminator":null,"CorporateFlag":0,"BEntID":249794,"AutID":227061,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Van Stappen","Firstname":"Gilbert","Initials":"G.","Affiliation":"Laboratory of Aquaculture & Artemia Reference Center, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":249794,"AutID":189073,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":null,"PersID":2565,"InsID":22},{"AutName":"Sorgeloos","Firstname":null,"Initials":"P.","Affiliation":"Univ Malaysia Terengganu, Fac Agrotechnol & Food Sci, Dept Fisheries & Aquaculture, Kuala Terengganu 21030, Malaysia.","Discriminator":null,"CorporateFlag":0,"BEntID":249794,"AutID":227063,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":35,"InsID":null},{"AutName":"Sung","Firstname":null,"Initials":"Y.","Affiliation":"Univ Malaysia Terengganu, Fac Agrotechnol & Food Sci, Dept Fisheries & Aquaculture, Kuala Terengganu 21030, Malaysia.","Discriminator":null,"CorporateFlag":0,"BEntID":249794,"AutID":227064,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"MacRae","Firstname":null,"Initials":"T.","Affiliation":"Dalhousie Univ, Dept Biol, Halifax, NS B3H 4J1, Canada.","Discriminator":null,"CorporateFlag":0,"BEntID":249794,"AutID":227066,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bossier","Firstname":"Peter","Initials":"P.","Affiliation":"Laboratory of Aquaculture & Artemia Reference Center, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":249794,"AutID":144468,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":"0000-0002-6165-9111","PersID":125,"InsID":22}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":341355,"LibID":36,"BRefID":257784,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"341355","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open Marien Archief","URL":null,"ClassifID":235,"Classification":"Open Marine Archive","ReqLink":null,"ClassifTypID":5,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":0,"InternalReq":0,"LoggedInReq":0,"Disclaimer":"Disclaimer_OMA","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Cambridge University Press","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Artemia"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":249794,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":249794,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1242/jeb.041772","externalID":"10.1242/jeb.041772","URLTypeCode":"DOI","URLID":47026,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Artemia","AphiaID":480245,"TaxtID":23717}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Artemia","DutchTerm":"Pekelkreeftjes","AphiaID":480245,"TaxtID":23717}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2016-05-29 23:30:20.550000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2019-12-23 08:08:21.843000","timezone_type":3,"timezone":"Europe/Brussels"}}}