{"refrec":{"BRefID":257856,"RR":"<b>Baudart, J.; Servais, P.; De Paoli, H.; Henry, A.; Lebaron, P.</b> (2009). Rapid enumeration of <i>Escherichia coli</i> in marine bathing waters: potential interference of nontarget bacteria. <i>J. Appl. Microbiol. 107(6)</i>: 2054-2062. <a href=\"http://dx.doi.org/10.1111/j.1365-2672.2009.04392.x\" target=\"_blank\">dx.doi.org/10.1111/j.1365-2672.2009.04392.x</a>","BEntID":249866,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Appl. Microbiol. 107(6)</i>: 2054-2062. <a href=\"https://dx.doi.org/10.1111/j.1365-2672.2009.04392.x\" target=\"_blank\">https://dx.doi.org/10.1111/j.1365-2672.2009.04392.x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Baudart, J.; Servais, P.; De Paoli, H.; Henry, A.; Lebaron, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Baudart, J. <i>et al.</i>","Englishabstract":"Aims: To compare the <i>Escherichia coli</i> quantification given by the ‘Coliplage<sup>®</sup>’ assay, based on the direct measurement of the ß-d-glucuronidase (GLUase) activity and the reference Most Probable Number (MPN) method from seawater sites and investigate the possible interference of non-<i>E. coli</i> strains in the GLUase activity measurement. Methods and Results: Comparison performed from 69 French coastal bathing sites (1401 samples) showed nonconcordance between both methods, only for 8% of samples. Non-<i>E. coli</i> 4-methylumbelliferyl-ß-d-glucuronide (MUG+) were isolated from nonconcordant samples. Phylogenetic analysis showed that <i>Gammaproteobacteria</i> were dominants and mainly represented by <i>Vibrio</i> species, which displayed GLUase activities on the same order of magnitude and sometimes much higher as <i>E. coli</i> reference strains. Conclusions: The‘Coliplage<sup>®</sup>’ assay is a rapid method for the quantification of <i>E. coli</i> showed few discordances with the standard MPN method. Some <i>Vibrio</i> species could interfere on the direct GLUase activity measurement of <i>E. coli</i>. Significance and Impact of the Study:  Data present the first qualitative investigation on disagreement between Coliplage<sup>®</sup> and the MPN results. If the interference of <i>Vibrio</i> species is confirmed <i>in situ</i>, appropriate treatments should be developed to remove the interfering signal.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Rapid enumeration of <i>Escherichia coli</i> in marine bathing waters: potential interference of nontarget bacteria","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"bathing waters; Escherichia coli; glucuronidase activity; real-timemonitoring; Vibrio","OtherDescriptors":null,"Notes":null,"AnaPub":2009,"MonPub":null,"DateUpdate":"2016-06-02","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000271785400031","VABBcode":null,"OpenAcc":1,"DOI":"10.1111/j.1365-2672.2009.04392.x"},"refs":null,"anarec":{"AnaID":257856,"PubliDate":2009,"Pagination":"2054-2062","XtraPublOfAnaID":null,"ISBN":null,"Volume":"107","Issue":"6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45067,"SerRR":"Journal of Applied Microbiology. Blackwell Science: Oxford.  ISSN 1364-5072; e-ISSN 1365-2672","StandardTitleSer":"Journal of Applied Microbiology","ISSN":"1364-5072","AbbrevSer":"J. Appl. Microbiol.","StandardTitleMon":null,"StartPage":2054,"Pages":9,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Baudart","Firstname":null,"Initials":"J.","Affiliation":"Univ Paris 06, UPMC, UMR7621, Lab Oceanog Biol Banyuls,Observat Oceanol, F-66651 Banyuls Sur Mer, France.","Discriminator":null,"CorporateFlag":0,"BEntID":249866,"AutID":227494,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Servais","Firstname":"Pierre","Initials":"P.","Affiliation":"Ecologie des Systèmes Aquatiques, Université Libre de Bruxelles","Discriminator":null,"CorporateFlag":0,"BEntID":249866,"AutID":138275,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ESA","InsFSN":"Université Libre de Bruxelles; École Interfacultaire de Bioingénieurs; Laboratoire d'Écologie des Systèmes Aquatiques","ORCID":null,"PersID":3194,"InsID":2404},{"AutName":"De Paoli","Firstname":null,"Initials":"H.","Affiliation":"Univ Paris 06, UPMC, UMR7621, Lab Oceanog Biol Banyuls,Observat Oceanol, F-66651 Banyuls Sur Mer, France.","Discriminator":null,"CorporateFlag":0,"BEntID":249866,"AutID":227496,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Henry","Firstname":null,"Initials":"A.","Affiliation":"Ctr Anal Environm, St Maurice, France.","Discriminator":null,"CorporateFlag":0,"BEntID":249866,"AutID":227497,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Lebaron","Firstname":null,"Initials":"P.","Affiliation":"Univ Paris 06, UPMC, UMR7621, Lab Oceanog Biol Banyuls,Observat Oceanol, F-66651 Banyuls Sur Mer, France.","Discriminator":null,"CorporateFlag":0,"BEntID":249866,"AutID":227498,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":291271,"LibID":36,"BRefID":257856,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"291271","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Blackwell Science","City":"Oxford"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Escherichia coli"},{"TaxTerm":"Vibrio"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":249866,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":249866,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1111/j.1365-2672.2009.04392.x","externalID":"10.1111/j.1365-2672.2009.04392.x","URLTypeCode":"DOI","URLID":47082,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Escherichia coli","AphiaID":422817,"TaxtID":23081},{"TaxTerm":"Vibrio","AphiaID":480248,"TaxtID":22617}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Escherichia coli","DutchTerm":null,"AphiaID":422817,"TaxtID":23081},{"TaxTerm":"Vibrio","DutchTerm":null,"AphiaID":480248,"TaxtID":22617}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2016-05-29 23:32:35.743000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2016-06-02 08:37:33.030000","timezone_type":3,"timezone":"Europe/Brussels"}}}