{"refrec":{"BRefID":257929,"RR":"<b>Phuoc, L.H.; Defoirdt, T.; Sorgeloos, P.; Bossier, P.</b> (2009). Virulence of luminescent and non-luminescent isogenic vibrios towards gnotobiotic <i>Artemia franciscana</i> larvae and specific pathogen-free <i>Litopenaeus vannamei</i> shrimp. <i>J. Appl. Microbiol. 106(4)</i>: 1388-1396. <a href=\"http://dx.doi.org/10.1111/j.1365-2672.2008.04107.x\" target=\"_blank\">dx.doi.org/10.1111/j.1365-2672.2008.04107.x</a>","BEntID":249939,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Appl. Microbiol. 106(4)</i>: 1388-1396. <a href=\"https://dx.doi.org/10.1111/j.1365-2672.2008.04107.x\" target=\"_blank\">https://dx.doi.org/10.1111/j.1365-2672.2008.04107.x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Phuoc, L.H.; Defoirdt, T.; Sorgeloos, P.; Bossier, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Phuoc, L.H. <i>et al.</i>","Englishabstract":"<b>Aims: </b> This study was conducted to test the virulence of luminescent (L) and non-luminescent (NL) isogenic strains of <i>Vibrio campbellii</i> LMG21363, <i>Vibrio harveyi</i> BB120 (wild type) and quorum-sensing mutant strains derived from the wild type such as <i>Vibrio harveyi</i> BB152, BB170, MM30 and BB886.</p></div><div class=\"para\"><p><b>Methods and Results: </b> The NL strains could be obtained by culturing rifampicin-resistant luminescent strains in the dark under static condition. The virulence of the L and NL strains was tested in gnotobiotic <i>Artemia franciscana</i> larvae challenged with 10<sup>4</sup> CFU ml<sup>-1</sup> of bacteria. All luminescent isogenic tested strains showed higher virulence compared to the NL strains. The virulence of L and NL <i>V. campbellii</i> and <i>V. harveyi</i> BB120 was also tested in specific pathogen-free juvenile shrimp upon intramuscular injection with 10<sup>6</sup> CFU of bacteria. In contrast with <i>Artemia</i>, there was no significant difference in mortality between the groups challenged with L and NL strains (<i>P </i>> 0·05). The non-luminescent strains were not able to revert back to the luminescent state and quorum sensing did not influence this phenotypic shift.</p></div><div class=\"para\"><p><b>Conclusions: </b> Luminescent <i>Vibrio</i> strains can switch to a non-luminescent state by culturing them in static conditions. The NL strains become less virulent as verified in <i>Artemia</i>.</p></div><div class=\"para\"><p><b>Significance and Impact of the Study: </b> The luminescent state of <i>Vibrio</i> cells in a culture needs to be verified in order to assure maintenance of virulence.</p>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Virulence of luminescent and non-luminescent isogenic vibrios towards gnotobiotic <i>Artemia franciscana</i> larvae and specific pathogen-free <i>Litopenaeus vannamei</i> shrimp","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"luminescent; non-luminescent; SPF shrimp; V; campbellii; V; harveyi","OtherDescriptors":null,"Notes":null,"AnaPub":2009,"MonPub":null,"DateUpdate":"2016-06-08","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000264022300035","VABBcode":null,"OpenAcc":1,"DOI":"10.1111/j.1365-2672.2008.04107.x"},"refs":null,"anarec":{"AnaID":257929,"PubliDate":2009,"Pagination":"1388-1396","XtraPublOfAnaID":null,"ISBN":null,"Volume":"106","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45067,"SerRR":"Journal of Applied Microbiology. Blackwell Science: Oxford.  ISSN 1364-5072; e-ISSN 1365-2672","StandardTitleSer":"Journal of Applied Microbiology","ISSN":"1364-5072","AbbrevSer":"J. Appl. 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