{"refrec":{"BRefID":258007,"RR":"<b>Brackman, G.; Defoirdt, T.; Miyamoto, C.; Bossier, P.; Van Calenbergh, S.; Nelis, H.J.; Coenye, T.</b> (2008). Cinnamaldehyde and cinnamaldehyde derivatives reduce virulence in <i>Vibrio</i> spp. by decreasing the DNA-binding activity of the quorum sensing response regulator LuxR. <i>BMC Microbiol. 8</i>. <a href=\"http://dx.doi.org/10.1186/1471-2180-8-149\" target=\"_blank\">dx.doi.org/10.1186/1471-2180-8-149</a>","BEntID":250017,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>BMC Microbiol. 8</i>. <a href=\"https://dx.doi.org/10.1186/1471-2180-8-149\" target=\"_blank\">https://dx.doi.org/10.1186/1471-2180-8-149</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Brackman, G.; Defoirdt, T.; Miyamoto, C.; Bossier, P.; Van Calenbergh, S.; Nelis, H.J.; Coenye, T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Brackman, G. <i>et al.</i>","Englishabstract":"BackgroundTo date, only few compounds targeting the AI-2 based quorum sensing (QS) system are known. In the present study, we screened cinnamaldehyde and substituted cinnamaldehydes for their ability to interfere with AI-2 based QS. The mechanism of QS inhibition was elucidated by measuring the effect on bioluminescence in several <i>Vibrio harveyi</i> mutants. We also studied <i>in vitro</i> the ability of these compounds to interfere with biofilm formation, stress response and virulence of <i>Vibrio</i> spp. The compounds were also evaluated in an <i>in vivo</i> assay measuring the reduction of <i>Vibrio harveyi</i> virulence towards <i>Artemia</i> shrimp.ResultsOur results indicate that cinnamaldehyde and several substituted derivatives interfere with AI-2 based QS without inhibiting bacterial growth. The active compounds neither interfered with the bioluminescence system as such, nor with the production of AI-2. Study of the effect in various mutants suggested that the target protein is LuxR. Mobility shift assays revealed a decreased DNA-binding ability of LuxR. The compounds were further shown to (i) inhibit biofilm formation in several <i>Vibrio</i> spp., (ii) result in a reduced ability to survive starvation and antibiotic treatment, (iii) reduce pigment and protease production in <i>Vibrio anguillarum</i> and (iv) protect gnotobiotic <i>Artemia</i> shrimp against virulent <i>Vibrio harveyi</i> BB120.ConclusionCinnamaldehyde and cinnamaldehyde derivatives interfere with AI-2 based QS in various <i>Vibrio</i> spp. by decreasing the DNA-binding ability of LuxR. The use of these compounds resulted in several marked phenotypic changes, including reduced virulence and increased susceptibility to stress. Since inhibitors of AI-2 based quorum sensing are rare, and considering the role of AI-2 in several processes these compounds may be useful leads towards antipathogenic drugs.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Cinnamaldehyde and cinnamaldehyde derivatives reduce virulence in <i>Vibrio</i> spp. by decreasing the DNA-binding activity of the quorum sensing response regulator LuxR","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2008,"MonPub":null,"DateUpdate":"2016-06-09","DateCreate":"2016-05-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000259868400003","VABBcode":null,"OpenAcc":1,"DOI":"10.1186/1471-2180-8-149"},"refs":null,"anarec":{"AnaID":258007,"PubliDate":2008,"Pagination":null,"XtraPublOfAnaID":null,"ISBN":null,"Volume":"8","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":212867,"SerRR":"BMC Microbiology. BioMed Central: London.  ISSN 1471-2180; e-ISSN 1471-2180","StandardTitleSer":"BMC Microbiology","ISSN":"1471-2180","AbbrevSer":"BMC Microbiol.","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Brackman","Firstname":"Gilles","Initials":"G.","Affiliation":"Laboratory of Pharmaceutical Microbiology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":250017,"AutID":229475,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"LPM","InsFSN":"Universiteit Gent; Faculteit Farmaceutische Wetenschappen; Vakgroep Farmaceutische Analyse; Laboratorium voor Farmaceutische Microbiologie","ORCID":null,"PersID":33998,"InsID":12025},{"AutName":"Defoirdt","Firstname":"Tom","Initials":"T.","Affiliation":"Laboratory of Microbial Ecology and Technology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":250017,"AutID":195177,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"CMET","InsFSN":"Ghent University; 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