{"refrec":{"BRefID":258055,"RR":"<b>Bruck, W.; Sennett, S.; Pomponi, S.; Willenz, P.; McCarthy, P.</b> (2008). Identification of the bacterial symbiont <i>Entotheonella</i> sp. in the mesohyl of the marine sponge <i>Discodermia</i> sp. <i>ISME J. 2(3)</i>: 335-339. <a href=\"http://dx.doi.org/10.1038/ismej.2007.91\" target=\"_blank\">dx.doi.org/10.1038/ismej.2007.91</a>","BEntID":250065,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":" <i>ISME J. 2(3)</i>: 335-339. <a href=\"https://dx.doi.org/10.1038/ismej.2007.91\" target=\"_blank\">https://dx.doi.org/10.1038/ismej.2007.91</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Bruck, W.; Sennett, S.; Pomponi, S.; Willenz, P.; McCarthy, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Bruck, W. <i>et al.</i>","Englishabstract":"The lithistid sponge <i>Discodermia dissoluta</i> (family Theonellidae), is found in deep-waters throughout the Caribbean sea and is the source of discodermolide, a natural product with potential anticancer properties, and other secondary metabolites. As with other sponges, large numbers of microbes are harbored in the sponge mesohyl. The microbial population of the sponge mesohyl shows an abundance of large filamentous microbes. Fractionation of the dissociated sponge allowed enrichment of this microbe, which was then identified by analysis of the 16S rRNA genes. Its identity was confirmed through the use of fluorescent <i>in situ</i> hybridization. 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