{"refrec":{"BRefID":258561,"RR":"Bouchard, J.N.; Yamasaki, H. (2009). Implication of nitric oxide in the heat-stress-induced cell death of the symbiotic alga Symbiodinium microadriaticum. Mar. Biol. (Berl.) 156(10): 2209-2220. http://dx.doi.org/10.1007/s00227-009-1249-3","BEntID":250570,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". Mar. Biol. (Berl.) 156(10): 2209-2220. http://dx.doi.org/10.1007/s00227-009-1249-3","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Bouchard, J.N.; Yamasaki, H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Bouchard, J.N.; Yamasaki, H.","Englishabstract":"One of the major consequences of global warming is a rise in sea surface temperature which may affect the survival of marine organisms including phytoplankton. Here, we provide experimental evidence for heat-induced cell death in a symbiotic microalga. Shifting Symbiodinium microadriaticum from 27 to 32°C resulted in an increase in mortality, an increase in caspase 3-like activity, and an increase in nitric oxide (NO) production. The caspase-like activity was strongly correlated with the production of NO in thermally challenged microalgae. For this experiment, the application of Ac-DEVD-CHO, a mammalian caspase 3-specific inhibitor, partly prevented (by 65%) the increase in caspase-like activity. To verify the relationship between NO and the caspase-like activity, S. microadriaticum were subsequently incubated with 1.0 mM of the following chemical NO donors: sodium nitroprusside (SNP), S-nitrosoglutathione (GSNO), S-nitroso-N-acetylpenicillamine (SNAP) and 3,3bis(Aminoethyl)-1-hydroxy-2-oxo-1-triazene (NOC-18). The supplementation of both SNP and NOC-18 caused a significant increase in caspase-like activity compared to the control treatment. Pre-treatment of the microalgae with the inhibitor Ac-DEVD-CHO before the supplementation of the different NO donors completely prevented the increase in caspase-like activity. These results suggest that NO could play a role in the induction of cell death in heat-stressed S. microadriaticum by mediating an increase in caspase-like activity.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Implication of nitric oxide in the heat-stress-induced cell death of the symbiotic alga Symbiodinium microadriaticum","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-15 01:32:52.581735","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2009,"MonPub":null,"DateUpdate":"2018-02-13","DateCreate":"2016-06-08","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000270180900001","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s00227-009-1249-3"},"refs":null,"anarec":{"AnaID":258561,"PubliDate":2009,"Pagination":"2209-2220","XtraPublOfAnaID":null,"ISBN":null,"Volume":"156","Issue":"10","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43352,"SerRR":"Marine Biology: International Journal on Life in Oceans and Coastal Waters. Springer: Heidelberg; Berlin. ISSN 0025-3162; e-ISSN 1432-1793","StandardTitleSer":"Marine Biology: International Journal on Life in Oceans and Coastal Waters","ISSN":"0025-3162","AbbrevSer":"Mar. Biol. (Berl.)","StandardTitleMon":null,"StartPage":2209,"Pages":12,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Bouchard","Firstname":"Josee","Initials":"J.N.","Affiliation":"Univ Ryukyus, Fac Sci, Okinawa 9030213, Japan.","Discriminator":null,"CorporateFlag":0,"BEntID":250570,"AutID":308889,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Yamasaki","Firstname":"Hideo","Initials":"H.","Affiliation":"Univ Ryukyus, Fac Sci, Okinawa 9030213, Japan.","Discriminator":null,"CorporateFlag":0,"BEntID":250570,"AutID":308890,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":291652,"LibID":36,"BRefID":258561,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"291652","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"
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