{"refrec":{"BRefID":258602,"RR":"<b>Steinke, D.; Zemlak, T.S.; Boutillier, J.A.; Hebert, P.D.N.</b> (2009). DNA barcoding of Pacific Canada’s fishes. <i>Mar. Biol. (Berl.) 156(12)</i>: 2641-2647. <a href=\"http://dx.doi.org/10.1007/s00227-009-1284-0\" target=\"_blank\">http://dx.doi.org/10.1007/s00227-009-1284-0</a>","BEntID":250610,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Biol. (Berl.) 156(12)</i>: 2641-2647. <a href=\"http://dx.doi.org/10.1007/s00227-009-1284-0\" target=\"_blank\">http://dx.doi.org/10.1007/s00227-009-1284-0</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Steinke, D.; Zemlak, T.S.; Boutillier, J.A.; Hebert, P.D.N.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Steinke, D. <i>et al.</i>","Englishabstract":"DNA barcoding—sequencing a standard region of the mitochondrial cytochrome c oxidase 1 gene (COI)—promises a rapid, accurate means of identifying animals to a species level. This study establishes that sequence variability in the barcode region permits discrimination of 98% of 201 fish species from the Canadian Pacific. The average sequence variation within species was 0.25%, while the average distance separating species within genera was 3.75%. The latter value was considerably lower than values reported in other studies, reflecting the dominance of the Canadian fauna by members of the young and highly diverse genus Sebastes. Although most sebastids possessed distinctive COI sequences, four species did not. As a partial offset to these cases, the barcode records indicated the presence of a new, broadly distributed species of Paraliparis and the possibility that Paraliparis pectoralis is actually a species pair. 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