{"refrec":{"BRefID":259861,"RR":"Herlory, O.; Richard, P.; Blanchard, G.F. (2007). Methodology of light response curves: application of chlorophyll fluorescence to microphytobenthic biofilms. Mar. Biol. (Berl.) 153(1): 91-101. http://dx.doi.org/10.1007/s00227-007-0787-9","BEntID":251872,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". Mar. Biol. (Berl.) 153(1): 91-101. http://dx.doi.org/10.1007/s00227-007-0787-9","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Herlory, O.; Richard, P.; Blanchard, G.F.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Herlory, O. et al.","Englishabstract":"The light response curve methodology for microphytobenthic biofilms was studied by comparing the two most usual approaches used in pulse amplitude modulated (PAM) fluorometry. The non-sequential light curve (N-SLC) method is characterized by independent measures of the photosynthetic activity across a light gradient whereas the rapid light curve (RLC) method consists of successive measures on the same sample exposed to a stepwise increase of light intensities. Experiments were carried out on experimental microphytobenthic biofilms prepared from natural assemblages and acclimated to dark conditions. In preliminary experiments, N-SLCs were constructed from fluorescence induction curves performed at 12 different photon flux densities (PFDs). A minimum of 50 s of illumination was necessary to reach a stable light response curve; shorter illumination times resulted in underestimating the physiological parameters (a the light utilization coefficient in light-limited conditions and rETRmax the maximum rate of photosynthesis efficiency) of the light response curve. For the comparison between N-SLCs and RLCs, the same time of illumination (50 s) was used for each light step of RLCs so that N-SLCs differed from RLCs only by the way the amount of light was delivered, i.e., a light dose accumulation for RLC. The experimental results showed the difference between the two photobiological response curves. In the lower range of PFDs, RLCs exhibited a larger value of a; in this light-limited part of the response curve the incremental increase of PFDs limited the development of NPQ and resulted in a better optimization of electron transport rate for RLC. In the higher range of PFDs, the trend was reversed and the RLC showed a lower value of rETRmax than the N-SLC did; this is attributed to the light dose accumulation which likely led to a more efficient dispersion of energy, as illustrated by a higher non-photochemical quenching (NPQ). In conclusion, these results confirm that parameters derived from both methods differ in their value and do not bear the same physiological information.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Methodology of light response curves: application of chlorophyll fluorescence to microphytobenthic biofilms","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-22 01:31:53.728984","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2007,"MonPub":null,"DateUpdate":"2016-07-07","DateCreate":"2016-07-07","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000250139700010","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s00227-007-0787-9"},"refs":null,"anarec":{"AnaID":259861,"PubliDate":2007,"Pagination":"91-101","XtraPublOfAnaID":null,"ISBN":null,"Volume":"153","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43352,"SerRR":"Marine Biology: International Journal on Life in Oceans and Coastal Waters. Springer: Heidelberg; Berlin. ISSN 0025-3162; e-ISSN 1432-1793","StandardTitleSer":"Marine Biology: International Journal on Life in Oceans and Coastal Waters","ISSN":"0025-3162","AbbrevSer":"Mar. Biol. (Berl.)","StandardTitleMon":null,"StartPage":91,"Pages":11,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Herlory","Firstname":null,"Initials":"O.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":251872,"AutID":234020,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Richard","Firstname":null,"Initials":"P.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":251872,"AutID":42976,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Blanchard","Firstname":null,"Initials":"G.F.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":251872,"AutID":29771,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":12774,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":293073,"LibID":36,"BRefID":259861,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"293073","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"
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