{"refrec":{"BRefID":26134,"RR":"<b>Vetrova, E.; Kratasyuk, V.; Kudryasheva, N.</b> (2002). Bioluminescence characteristics of Lake Shira water. <i>Aquat. Ecol. 36(2)</i>: 309-315","BEntID":26134,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Ecol. 36(2)</i>: 309-315","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Vetrova, E.; Kratasyuk, V.; Kudryasheva, N.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Vetrova, E.; Kratasyuk, V.; Kudryasheva, N.","Englishabstract":"Bioluminescence bioassays based on luminous bacteria (<i>Photobacterium phosphopreum</i>) and coupled enzyme system NADH-FMN-oxidoreductase-luciferase were adapted for monitoring the saline-water conditions of Lake Shira (Khakasia, Siberia). The differences in bioluminescence responses have been found to be related to the salt composition and the oxidation-reduction properties of water. Bioluminescent kinetics parameters, which are mostly sensitive to pollution under conditions of saline water, have been observed. The enzymatic system in the presence of 1,4-benzoquinone are shown to be more sensitive to redox characteristics of the salt water than this in the absence of 1,4-benzoquinone. 1,4-benzoquinone should be applied for the preparation of a model solution for the monitoring of redox properties of the salt water. Using this technique, the results of bioluminescence analysis are used to construct a heterogeneity map that characterizes the spatial and temporal water quality of lake Shira. A partial map was based on the bioluminescence characteristics of water samples taken along the shoreline, sampling stations in the different places and in different depths of the lake. 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