{"refrec":{"BRefID":261472,"RR":"<b>Scheerer, S.; Gomez, F.; Lloyd, D.</b> (2006). Bioluminescence of <i>Vibrio fischeri</i> in continuous culture: Optimal conditions for stability and intensity of photoemission. <i>J. microbiol. methods 67(2)</i>: 321-329. <a href=\"http://dx.doi.org/10.1016/j.mimet.2006.04.010\" target=\"_blank\">http://dx.doi.org/10.1016/j.mimet.2006.04.010</a>","BEntID":253490,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. microbiol. methods 67(2)</i>: 321-329. <a href=\"http://dx.doi.org/10.1016/j.mimet.2006.04.010\" target=\"_blank\">http://dx.doi.org/10.1016/j.mimet.2006.04.010</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Scheerer, S.; Gomez, F.; Lloyd, D.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Scheerer, S. <i>et al.</i>","Englishabstract":"Continuous cultivation of the bioluminescent bacterium, <i>Vibrio fischeri</i> NRRL-B-11177, in a fermenter provided a constant supply of bacteria in exponential growth phase. These bacteria may be used as stable bioindicators to measure perturbation of the metabolic state by physical and chemical agents. However, optimization of light emission necessitates careful choice of growth medium and culture operating conditions. Optimized conditions that supported long-term cultivation of <i>V. fischeri</i> NRRL-B-11177 with stable intense bioluminescence were: a dilution rate of 0.08-0.09 h<sup>-1</sup>, air supply of 600 ml<sup>-1</sup>, stirring of 300-350 rpm at a constant incubation temperature within the range of 20 to 26 °C and pH 7.8. These conditions were as successful in a 500-ml as in a 10-ml fermenter. 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