{"refrec":{"BRefID":261531,"RR":"<b>Zhang, Q.; You, C.; Wang, S.; Dong, Y.; Monroig, Ó.; Tocher, D.R.; Li, Y.</b> (2016). The miR-33 gene is identified in a marine teleost: a potential role in regulation of LC-PUFA biosynthesis in <i>Siganus canaliculatus</i>. <i>NPG Scientific Reports 6(32909 )</i>: 10 pp. <a href=\"https://dx.doi.org/10.1038/srep32909\" target=\"_blank\">https://dx.doi.org/10.1038/srep32909</a>","BEntID":253549,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 6(32909 )</i>: 10 pp. <a href=\"https://dx.doi.org/10.1038/srep32909\" target=\"_blank\">https://dx.doi.org/10.1038/srep32909</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Zhang, Q.; You, C.; Wang, S.; Dong, Y.; Monroig, Ó.; Tocher, D.R.; Li, Y.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Zhang, Q. <i>et al.</i>","Englishabstract":"As the first marine teleost demonstrated to have the ability to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFA) from C-18 PUFA precursors, rabbitfish Siganus canaliculatus provides a good model for studying the regulatory mechanisms of LC-PUFA biosynthesis in teleosts. Here the potential roles of miR-33 in such regulation were investigated. The miR-33 gene was identified within intron 16 of the gene encoding sterol regulatory element-binding protein 1 (Srebp1), an activator of LC-PUFA biosynthesis. Expression of miR-33 in rabbitfish tissues correlated with that of srebp1, while its expression in liver was highly responsive to ambient salinities and PUFA components, factors affecting LC-PUFA biosynthesis. Srebp1 activation promoted the expression of Delta 4 and Delta 6 Delta 5 fatty acyl desaturases (Fad), key enzymes for LC-PUFA biosynthesis, accompanied by elevated miR-33 abundance in rabbitfish hepatocytes. miR-33 overexpression induced the expression of the two fad, but suppressed that of insulin-induced gene 1 (insig1), which encodes a repressor blocking Srebp proteolytic activation and has targeting sites of miR-33. These results indicated that miR-33, cooperating with Srebp1, may be involved in regulation of LC-PUFA biosynthesis by facilitating fad expression, probably through targeting insig1. To our knowledge, this is the first report of the participation of miR-33 in LC-PUFA biosynthesis in vertebrates.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The miR-33 gene is identified in a marine teleost: a potential role in regulation of LC-PUFA biosynthesis in <i>Siganus canaliculatus</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-12 01:32:58.335699","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2018-02-13","DateCreate":"2016-09-22","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000383323000001","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/srep32909"},"refs":null,"anarec":{"AnaID":261531,"PubliDate":2016,"Pagination":"10 pp","XtraPublOfAnaID":null,"ISBN":null,"Volume":"6","Issue":"32909 ","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). 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