{"refrec":{"BRefID":261740,"RR":"<b>Devreese, B.; Vanrobaeys, F.; Van Beeumen, J.</b> (2001). Automated nanoflow liquid chromatography/tandem mass spectrometric identification of proteins from <i>Shewanella putrefaciens</i> separated by two-dimensional polyacrylamide gel electrophoresis. <i>Rapid Comm. Mass Spectrom. 15(1)</i>: 50-56. <a href=\"https://dx.doi.org/10.1002/1097-0231(20010115)15:1<50::AID-RCM191>3.0.CO;2-V\" target=\"_blank\">https://dx.doi.org/10.1002/1097-0231(20010115)15:1<50::AID-RCM191>3.0.CO;2-V</a>","BEntID":253758,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Rapid Comm. Mass Spectrom. 15(1)</i>: 50-56. <a href=\"https://dx.doi.org/10.1002/1097-0231(20010115)15:1<50::AID-RCM191>3.0.CO;2-V\" target=\"_blank\">https://dx.doi.org/10.1002/1097-0231(20010115)15:1<50::AID-RCM191>3.0.CO;2-V</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Devreese, B.; Vanrobaeys, F.; Van Beeumen, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Devreese, B. <i>et al.</i>","Englishabstract":"The implementation of nanoflow liquid chromatography offers unique opportunities for automation of proteomics research. We demonstrate that automated nanoflow LC/MS/MS allowed the unambiguous identification of proteins from the omnipotent bacterium <i>Shewanella putrefaciens</i>, based on similarity searches against the completely determined genome of related microorganisms and against non-redundant databases. Total protein extracts were separated by 2-dimensional polyacrylamide electrophoresis. Only 1/20th of a tryptic digest mixture obtained from a single Coomassie Blue stained spot was loaded on the nanoflow LC column using a preconcentration/desalting step, and analyzed on-line on a hybrid quadrupole time-of-flight mass spectrometer with an automated MS-to-MS/MS switching protocol. This method allowed the <i>de novo</i> peptide sequence determination of several tryptic fragments and the identification of different proteins.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Automated nanoflow liquid chromatography/tandem mass spectrometric identification of proteins from <i>Shewanella putrefaciens</i> separated by two-dimensional polyacrylamide gel electrophoresis","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:01.897972","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2001,"MonPub":null,"DateUpdate":"2018-08-14","DateCreate":"2016-10-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000166425400008","VABBcode":null,"OpenAcc":0,"DOI":"10.1002/1097-0231(20010115)15:1<50::AID-RCM191>3.0.CO;2-V"},"refs":null,"anarec":{"AnaID":261740,"PubliDate":2001,"Pagination":"50-56","XtraPublOfAnaID":null,"ISBN":null,"Volume":"15","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":228889,"SerRR":"Rapid Communications in Mass Spectrometry. 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