{"refrec":{"BRefID":261852,"RR":"<b>Zapata, O.; Ralston, J.; Beltran, C.; Parys, J.B.; Chen, J.L.; Longo, F.J.; Darszon, A.</b> (1997). Inositol triphosphate receptors in sea urchin sperm. <i>Zygote 5(4)</i>: 355-364. <a href=\"https://dx.doi.org/10.1017/S0967199400003932\" target=\"_blank\">https://dx.doi.org/10.1017/S0967199400003932</a>","BEntID":253870,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Zygote 5(4)</i>: 355-364. <a href=\"https://dx.doi.org/10.1017/S0967199400003932\" target=\"_blank\">https://dx.doi.org/10.1017/S0967199400003932</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Zapata, O.; Ralston, J.; Beltran, C.; Parys, J.B.; Chen, J.L.; Longo, F.J.; Darszon, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Zapata, O. <i>et al.</i>","Englishabstract":"Inositol 1,4,5-triphosphate (Ins(1,4,5)P<sub>3</sub>) is a second messenger that regulates Ca<sup>2+</sup> channels in many important cell signalling pathways. In sea urchin sperm the outer investment of the egg triggers the acrosome reaction (AR) that involves Ins(1,4,5)P<sub>3</sub> production and the opening of two Ca<sup>2+</sup> channels. Here we have sought to identify a high-affinity Ins(1,4,5)P<sub>3</sub> receptor in <i>Strongylocentrotus purpuratus</i> sperm. An Ins(1,4,5)P<sub>3</sub> binding component was affinity-purified 12-fold from sperm extracts. It displayed similar characteristics to the Ins(1,4,5)P<sub>3</sub> receptor from other sources: pH-dependent high affinity for Ins(1,4,5)<sub>3</sub>(K<sub>D</sub>=261 nM), a τ<sub>1/2</sub> of association and dissociation of 50 and 40s, respectively, specificity (IC)<sub>50>5μM</sub> for Ins (1)P<sub>1</sub>, Ins(1,4)P<sub>2</sub> and Ins(1,3,4,5 P<sub>4</sub>), and pharmacological sensitivity 10 and 100μ heparin/ml inhibited 75% and 100% binding respectively). An antibody against the carboxy-terminal of the type I Ins(1,4,5)P<sub>3</sub> receptor of somatic cells recognised a Plasma membrane component in the sperm head and less intensely in the flagella. This antibody also recongnised a 240 kDa band from isolated head plasma membranes, and weakly in flagellar membrane. This IP<sub>3</sub> receptor-like protein may mediate the sustained uptake of Ca<sup>2+</sup> through the second Ca<sup>2+</sup> chanel opened during the AR.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Inositol triphosphate receptors in sea urchin sperm","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"acrosome reaction; Ca(2+) channels; Ins(1,4,5)P(3); intracellularCa(2+); sea urchin sperm","OtherDescriptors":null,"Notes":null,"AnaPub":1997,"MonPub":null,"DateUpdate":"2022-04-13","DateCreate":"2016-10-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000073939200007","VABBcode":null,"OpenAcc":0,"DOI":"10.1017/S0967199400003932"},"refs":null,"anarec":{"AnaID":261852,"PubliDate":1997,"Pagination":"355-364","XtraPublOfAnaID":null,"ISBN":null,"Volume":"5","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":280349,"SerRR":"Zygote. CAMBRIDGE UNIV PRESS: New York.  ISSN 0967-1994; e-ISSN 1469-8730","StandardTitleSer":"Zygote","ISSN":"0967-1994","AbbrevSer":null,"StandardTitleMon":null,"StartPage":355,"Pages":10,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Zapata","Firstname":null,"Initials":"O.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":253870,"AutID":238979,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Ralston","Firstname":null,"Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":253870,"AutID":238980,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Beltran","Firstname":null,"Initials":"C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":253870,"AutID":238981,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Parys","Firstname":null,"Initials":"J.B.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":253870,"AutID":238982,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Chen","Firstname":null,"Initials":"J.L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":253870,"AutID":238983,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Longo","Firstname":null,"Initials":"F.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":253870,"AutID":238984,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Darszon","Firstname":null,"Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":253870,"AutID":238985,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":375442,"LibID":36,"BRefID":261852,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"375442","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"CAMBRIDGE UNIV PRESS","City":"New York"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":253870,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":253870,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1017/S0967199400003932","externalID":"10.1017/S0967199400003932","URLTypeCode":"DOI","URLID":102212,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2016-10-03 20:16:18.820000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2022-04-13 08:50:49.890000","timezone_type":3,"timezone":"Europe/Brussels"}}}