{"refrec":{"BRefID":261887,"RR":"<b>Czerwiec, E.; De Backer, J.-P.; Vauquelin, G.; Vanderheyden, P.M.L.</b> (1996). Neuropeptide Y receptors from calf brain: effect of crude <i>Conus</i> venom preparations on [<sup>3</sup>]NPY binding. <i>Neurochemistry International 29(6)</i>: 669-676. <a href=\"https://dx.doi.org/10.1016/S0197-0186(96)00066-6\" target=\"_blank\">https://dx.doi.org/10.1016/S0197-0186(96)00066-6</a>","BEntID":253905,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Neurochemistry International 29(6)</i>: 669-676. <a href=\"https://dx.doi.org/10.1016/S0197-0186(96)00066-6\" target=\"_blank\">https://dx.doi.org/10.1016/S0197-0186(96)00066-6</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Czerwiec, E.; De Backer, J.-P.; Vauquelin, G.; Vanderheyden, P.M.L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Czerwiec, E. <i>et al.</i>","Englishabstract":"NPY receptors are identified in calf frontal cortex and hippocampus membrane preparations by binding of <i>N</i>-[propionyl-<sup>3</sup>H] neuropeptide Y. Saturation and competition binding data with PYY, NPY-(18–36) and NPY itself fit with a single class of sites: for the radioligand <i>K</i><sub>D</sub> = 1.4 ± 0.5 nM, <i>B</i><sub>max</sub> = 434 ± 180 fmol/mg protein in frontal cortex, <i>K</i><sub>D</sub> = 0.7 ± 0.2 nM, <i>B</i><sub>max</sub> = 267 ± 50 fmol/mg protein in hippocampus. Competition curves of the Y<sub>1</sub>-subtype selective agonist [Leu<sup>31</sup>, Pro<sup>34</sup>]NPY are biphasic in both membrane preparations: high affinity sites (i.e. Y<sub>1</sub>-subtype) amount to 80% in frontal cortex and 23% in hippocampus. The remaining sites are of the Y<sub>2</sub>-subtype. Out of 23 <i>Conus</i> venom preparations, 17 inhibit the binding of [<sup>3</sup>H]NPY in both membrane preparations, but only two of them (from <i>Conus aulicus</i> and <i>C. pennaceus</i>) do so with high potency (ic<sub>50</sub> < 5 μg protein/ml). Only one venom preparation (from <i>C. mercator</i>) had weak discriminatory properties (ic<sub>50</sub>Y<sub>2</sub>/ic<sub>50</sub>Y<sub>1</sub> = 6). Venom from <i>C. anemone</i> increased the [<sup>3</sup>H]NPY binding 5-fold and with an ic<sub>50</sub> of 15–18 μg protein/ml. This binding occurred to the venom itself and was unrelated to the NPY receptors since it was equally potent when displaced by [Leu<sup>31</sup>, Pro<sup>34</sup>]NPY, NPY-(18–36), PYY and NPY.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Neuropeptide Y receptors from calf brain: effect of crude <i>Conus</i> venom preparations on [<sup>3</sup>]NPY binding","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1996,"MonPub":null,"DateUpdate":"2022-07-13","DateCreate":"2016-10-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1996VY60800012","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/S0197-0186(96)00066-6"},"refs":null,"anarec":{"AnaID":261887,"PubliDate":1996,"Pagination":"669-676","XtraPublOfAnaID":null,"ISBN":null,"Volume":"29","Issue":"6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":275301,"SerRR":"Neurochemistry International. 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