{"refrec":{"BRefID":261891,"RR":"<b>Genicot, S.; Rentier-Delrue, F.; Edwards, D.; Van Beeumen, J.; Gerday, C.</b> (1996). Trypsin and trypsinogen from an Antarctic fish: molecular basis of cold adaptation. <i>Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1298(1)</i>: 45-57. <a href=\"https://dx.doi.org/10.1016/S0167-4838(96)00095-7\" target=\"_blank\">https://dx.doi.org/10.1016/S0167-4838(96)00095-7</a>","BEntID":253909,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1298(1)</i>: 45-57. <a href=\"https://dx.doi.org/10.1016/S0167-4838(96)00095-7\" target=\"_blank\">https://dx.doi.org/10.1016/S0167-4838(96)00095-7</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Genicot, S.; Rentier-Delrue, F.; Edwards, D.; Van Beeumen, J.; Gerday, C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Genicot, S. <i>et al.</i>","Englishabstract":"Trypsin from Antarctic fish <i>Paranotothenia magellanica</i> displays molecular and kinetic properties typical of enzymes produced by psychrophilic organisms. The enzyme has a high catalytic efficiency at low and moderate temperatures and is rapidly inactivated at temperatures higher than 30°C. The nucleotide sequence was determined after mRNA extraction and cDNA synthesis. The cDNA encodes a pretrypsinogen which includes a seven residue activation peptide containing only three acidic residues preceeding the 222 amino-acid mature enzyme. A three-dimensional model of the enzyme was built. Structural parameters possibly involved in the adaptation to cold have been derived from comparison with the three-dimensional structure of the bovine enzyme. Among them are the lack of Tyr-151 in the substrate binding pocket, an overall decrease in the number of salt bridges and hydrophobicity and the increase in the surface hydrophilicity.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Trypsin and trypsinogen from an Antarctic fish: molecular basis of cold adaptation","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"trypsin; cold adaptation; Antarctic fish; three-dimensional structure;(P-magellanica)","OtherDescriptors":null,"Notes":null,"AnaPub":1996,"MonPub":null,"DateUpdate":"2022-07-14","DateCreate":"2016-10-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1996VV01200008","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/S0167-4838(96)00095-7"},"refs":null,"anarec":{"AnaID":261891,"PubliDate":1996,"Pagination":"45-57","XtraPublOfAnaID":null,"ISBN":null,"Volume":"1298","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":258648,"SerRR":"Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 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